Rabbit anti-Human, Mouse Angiotensinogen Polyclonal Antibody | anti-AGT antibody
Rabbit anti-human Angiotensinogen polyclonal Antibody(AGT)
IHC:1:20-1:200
Western Blot (WB)
(All lanes: Angiotensinogen antibody at 3ug/ml+mouse liver tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 53kDaObserved band size: 53kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human kidney using MBS1498076 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunofluorescent analysis of HepG2 cells using MBS1498076 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq, Jul 2008]
Uniprot Description
angiotensin: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1- 8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2. Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT). Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause. Defects in AGT are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Belongs to the serpin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q42.2
Cellular Component: cytoplasm; extracellular region; extracellular space
Molecular Function: growth factor activity; hormone activity; protein binding; serine-type endopeptidase inhibitor activity; sodium channel regulator activity; superoxide-generating NADPH oxidase activator activity; type 1 angiotensin receptor binding; type 2 angiotensin receptor binding
Biological Process: activation of NF-kappaB transcription factor; activation of NF-kappaB-inducing kinase; aging; angiotensin maturation; angiotensin mediated drinking behavior; angiotensin mediated regulation of renal output; angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure; artery smooth muscle contraction; astrocyte activation; blood vessel development; blood vessel remodeling; cell surface receptor linked signal transduction; cell-cell signaling; cell-matrix adhesion; cellular lipid metabolic process; cellular protein metabolic process; cellular sodium ion homeostasis; cytokine secretion; establishment of blood-nerve barrier; excretion; extracellular matrix organization and biogenesis; female pregnancy; fibroblast proliferation; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cGMP nucleotide second messenger; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); kidney development; negative regulation of angiogenesis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of nerve growth factor receptor signaling pathway; negative regulation of neuron apoptosis; negative regulation of tissue remodeling; nitric oxide mediated signal transduction; ovarian follicle rupture; peristalsis; phospholipase C activation; positive regulation of cellular protein metabolic process; positive regulation of cytokine production; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of fatty acid biosynthetic process; positive regulation of fibroblast proliferation; positive regulation of inflammatory response; positive regulation of MAPKKK cascade; positive regulation of multicellular organism growth; positive regulation of NAD(P)H oxidase activity; positive regulation of nitric oxide biosynthetic process; positive regulation of organ growth; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of superoxide release; positive regulation of transcription, DNA-dependent; positive regulation of vasodilation; regulation of blood pressure; regulation of calcium ion transport; regulation of cell growth; regulation of cell proliferation; regulation of heart rate; regulation of long-term neuronal synaptic plasticity; regulation of norepinephrine secretion; regulation of transmission of nerve impulse; regulation of vasoconstriction; renal response to blood flow during renin-angiotensin regulation of systemic arterial blood pressure; renal system process; renin-angiotensin regulation of aldosterone production; renin-angiotensin regulation of blood vessel size; renin-angiotensin regulation of blood volume; response to cold; response to muscle activity involved in regulation of muscle adaptation; response to salt stress; smooth muscle cell differentiation; smooth muscle cell proliferation; stress-activated MAPK cascade; ureteric bud branching; vasodilation
Disease: Hypertension, Essential; Renal Tubular Dysgenesis
Research Articles on AGT
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Product Notes
The AGT agt (Catalog #AAA1498076) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Angiotensinogen polyclonal Antibody(AGT) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Angiotensinogen can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the AGT agt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiotensinogen, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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