Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Angiopoietin 4 (ANGPT4) Polyclonal Antibody | anti-ANGPT4 antibody
Polyclonal Antibody to Angiopoietin 4 (ANGPT4)
Gene Names
Ang4; Raa4; Rnase5d
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Angiopoietin 4 (ANGPT4); Polyclonal Antibody; Polyclonal Antibody to Angiopoietin 4 (ANGPT4); anti-ANGPT4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-ANGPT4 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2 ug/mL
Immunohistochemistry: 5-20 ug/mL
Immunocytochemistry: 5-20 ug/mL
Optimal working dilutions must be determined by end user
Immunohistochemistry: 5-20 ug/mL
Immunocytochemistry: 5-20 ug/mL
Optimal working dilutions must be determined by end user
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant ANGPT4 (Phe32~Pro144 (Accession # Q3TMQ6)) expressed in E.coli
Cross Reactivity
Mouse
Preparation and Storage
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
angiogenin-4
NCBI Official Synonym Full Names
angiogenin, ribonuclease A family, member 4
NCBI Official Symbol
Ang4
NCBI Official Synonym Symbols
Raa4; Rnase5d
NCBI Protein Information
angiogenin-4
UniProt Protein Name
Angiogenin-4
Protein Family
UniProt Gene Name
Ang4
Uniprot Description
ANG4: Has bactericidal activity against E.faecalis and L.monocytogenes, but not against L.innocua and E.coli. Promotes angiogenesis (in vitro). Has low ribonuclease activity (in vitro). Promotes proliferation of melanoma cells, but not of endothelial cells or fibroblasts (in vitro). Belongs to the pancreatic ribonuclease family.
Protein type: EC 3.1.27.-
Chromosomal Location of Human Ortholog: 14|14 C1
Cellular Component: cytoplasmic vesicle; extracellular region; extracellular space; nucleus; secretory granule
Molecular Function: endonuclease activity; hydrolase activity; nuclease activity; nucleic acid binding; ribonuclease activity
Biological Process: angiogenesis; antibacterial humoral response; defense response to bacterium; positive regulation of cell proliferation; RNA catabolic process
Research Articles on ANGPT4
Similar Products
Product Notes
The ANGPT4 ang4 (Catalog #AAA2006130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Angiopoietin 4 (ANGPT4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Angiopoietin 4 (ANGPT4) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP). Western blotting: 0.5-2 ug/mL Immunohistochemistry: 5-20 ug/mL Immunocytochemistry: 5-20 ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ANGPT4 ang4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiopoietin 4 (ANGPT4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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