Western Blot (WB)
(Western blot analysis of Angiopoietin-1 expression in MCF7 (A), HeLa (B), U87MG (C) whole cell lysates.)
Rabbit Angiopoietin-1 Polyclonal Antibody | anti-ANGPT1 antibody
Anti-Angiopoietin-1 Antibody
Western Blot (WB)
(Western blot analysis of Angiopoietin-1 expression in MCF7 (A), HeLa (B), U87MG (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Angiopoietin-1 expression in MCF7 (A), HeLa (B), U87MG (C) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Angiopoietin-1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of Angiopoietin-1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]
Uniprot Description
ANGPT1: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 8q23.1
Cellular Component: extracellular region; extracellular space; intracellular; lipid raft; microvillus; plasma membrane
Molecular Function: protein binding; receptor tyrosine kinase binding
Biological Process: activation of MAPKK activity; axon guidance; blood coagulation; cell-substrate adhesion; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; hemopoiesis; heparin biosynthetic process; in utero embryonic development; innate immune response; insulin receptor signaling pathway; leukocyte migration; MAPKKK cascade; negative regulation of apoptosis; negative regulation of cell adhesion; negative regulation of cytokine secretion during immune response; negative regulation of neuron apoptosis; negative regulation of protein amino acid phosphorylation; negative regulation of protein import into nucleus; negative regulation of vascular permeability; nerve growth factor receptor signaling pathway; positive chemotaxis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell adhesion; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of protein ubiquitination; positive regulation of receptor internalization; Ras protein signal transduction; regulation of endothelial cell proliferation; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of protein binding; regulation of satellite cell proliferation; regulation of tumor necrosis factor production; small GTPase mediated signal transduction; sprouting angiogenesis; Tie receptor signaling pathway; transmembrane receptor protein tyrosine kinase activation (dimerization); vascular endothelial growth factor receptor signaling pathway
Research Articles on ANGPT1
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Product Notes
The ANGPT1 angpt1 (Catalog #AAA8241801) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Angiopoietin-1 Antibody reacts with Human, Mouse, Rat, Bovine, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Angiopoietin-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: (1/500 - 1/1000); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500). Researchers should empirically determine the suitability of the ANGPT1 angpt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiopoietin-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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