Rabbit anti-Human AMPK gamma 2 Polyclonal Antibody | anti-Prkag2 antibody

AMPK gamma 2 (5'-AMP-activated protein kinase, gamma-2 subunit, PRKAG2)

Cat. Num. AAA614412

• Gene Names: Prkag2; Prkag2
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: AMPK gamma 2; Polyclonal Antibody; AMPK gamma 2 (5'-AMP-activated protein kinase; gamma-2 subunit; PRKAG2); Anti -AMPK gamma 2 (5'-AMP-activated protein kinase; anti-Prkag2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Reacts with the N-terminal region of the human AMPK 2 protein. On Western blots, it identifies a target band at ~63kD for human and ~62kD for mouse. Reactivity has been confirmed with human NGP96 neuroblastoma lysates, JEG-3 human placenta choriocarcinoma lysates, and mouse liver homogenates.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-Prkag2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-3ug/ml
• Immunogen: Synthetic peptide derived from the N-terminal region of the human AMPK gamma 2 (5'-AMP-activated protein kinase, gamma-2 subunit, PRKAG2) protein, which differs from mouse by one amino acid.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Prkag2 antibody

5 -AMP-activated Protein Kinase (AMPK, also known as the heart-abundant protein PRKAG21), plays a key role in the regulation of energy homeostasis. AMPK acts as a metabolic stress-inducing protein kinase that switches off biosynthetic pathways when cellular ATP levels are depleted, and when 5`-AMP rises in response to fuel limitation and/or hypoxia. AMPK is activated by high AMP and low ATP via a mechanism that involves allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase (AMPKK), and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. AMPK may also increase the

Product Categories/Family for anti-Prkag2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 34576559
• NCBI GeneID: 373545
• NCBI Accession #: NP_908940.1
• NCBI GenBank Nucleotide #: NM_184051.1
• UniProt Accession #: Q6V7V4; Q4QRB9; Q6U7I6
• Molecular Weight: 37,311 Da
• NCBI Official Full Name: 5'-AMP-activated protein kinase subunit gamma-2
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, gamma 2 non-catalytic subunit
• NCBI Official Symbol: Prkag2
• NCBI Official Synonym Symbols: Prkag2
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit gamma-2; AMPK gamma 2; AMP-activated protein kinase gamma2 subunit; adenosine monophosphate-activated protein kinase gamma 2-subunit
• UniProt Protein Name: AMP-activated protein kinase gamma 2 non-catalytic subunit
• Protein Family: 5'-AMP-activated protein kinase
• UniProt Gene Name: Prkag2
• UniProt Entry Name: Q6V7V4_RAT

NCBI Description

predicted to act as a non-catalytic subunit of an AMP-activated protein kinase [RGD]

Uniprot Description

AMPKG2: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non- catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2. Isoform B is ubiquitously expressed except in liver and thymus. The highest level is detected in heart with abundant expression in placenta and testis. Belongs to the 5'-AMP-activated protein kinase gamma subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, regulatory subunit; Autophagy

Cellular Component: extracellular space; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; ADP binding; phosphorylase kinase regulator activity; protein kinase binding; protein kinase activator activity; ATP binding; AMP binding

Biological Process: glycogen metabolic process; regulation of catalytic activity; regulation of glycolysis; positive regulation of protein kinase activity; regulation of fatty acid metabolic process; negative regulation of protein kinase activity; protein amino acid phosphorylation