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Western Blot (WB) (Western analysis of recombinant human Amphiregulin using a rabbit anti-human Amphiregulin polyclonal antibody.)

Rabbit anti-Human Amphiregulin Polyclonal Antibody | anti-AREG antibody

Rabbit Anti-Human Amphiregulin

Gene Names
AREG; AR; SDGF; AREGB; CRDGF
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Synonyms
Amphiregulin; Polyclonal Antibody; Rabbit Anti-Human Amphiregulin; AREG; AR; SDGF; AREGB; CRDGF; anti-AREG antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Form/Format
Lyophilized
Sequence Length
252
Applicable Applications for anti-AREG antibody
Western Blot (WB), Immunofluorescence (IF)
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Antigen
Recombinant Human Amphiregulin
Preparation and Storage
The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C.

Western Blot (WB)

(Western analysis of recombinant human Amphiregulin using a rabbit anti-human Amphiregulin polyclonal antibody.)

Western Blot (WB) (Western analysis of recombinant human Amphiregulin using a rabbit anti-human Amphiregulin polyclonal antibody.)

Immunofluorescence (IF)

(Immunofluorescence staining of human Amphiregulin in a mixture of primary human endothelial cells (HDLEC, HUVEC) and primary normal human dermal firbroblast (NHDF) with a polyclonal rabbit anti-human Amphiregulin antibody. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) [Dianova].)

Immunofluorescence (IF) (Immunofluorescence staining of human Amphiregulin in a mixture of primary human endothelial cells (HDLEC, HUVEC) and primary normal human dermal firbroblast (NHDF) with a polyclonal rabbit anti-human Amphiregulin antibody. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) [Dianova].)

Immunofluorescence (IF)

(Immunofluorescence staining of human Amphiregulin in primary normal human dermal fibroblasts (NHDF) and primary human dermal lymphatic endothelial cells (HDLEC) with a polyclonal rabbit anti-human Amphiregulin antibody. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) Dianova].)

Immunofluorescence (IF) (Immunofluorescence staining of human Amphiregulin in primary normal human dermal fibroblasts (NHDF) and primary human dermal lymphatic endothelial cells (HDLEC) with a polyclonal rabbit anti-human Amphiregulin antibody. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) Dianova].)

Immunofluorescence (IF)

(Immunofluorescence staining (cryo-sections) of vasa vasorum of a human lymphatic collector. (fixed 15 min in 4% PFA) with anti-human Amphiregulin (5ug/ml) and counter staining of nuclei with Dapi. [400X] The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.)

Immunofluorescence (IF) (Immunofluorescence staining (cryo-sections) of vasa vasorum of a human lymphatic collector. (fixed 15 min in 4% PFA) with anti-human Amphiregulin (5ug/ml) and counter staining of nuclei with Dapi. [400X] The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.)
Related Product Information for anti-AREG antibody
Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulins extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant human Amphiregulin is a 11.3 kDa glycoprotein consisting of 98 amino acid residues.
Product Categories/Family for anti-AREG antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
374
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,895 Da
NCBI Official Full Name
amphiregulin preproprotein
NCBI Official Synonym Full Names
amphiregulin
NCBI Official Symbol
AREG
NCBI Official Synonym Symbols
AR; SDGF; AREGB; CRDGF
NCBI Protein Information
amphiregulin
UniProt Protein Name
Amphiregulin
Protein Family
UniProt Gene Name
AREG
UniProt Synonym Gene Names
CRDGF
UniProt Entry Name
AREG_HUMAN

Uniprot Description

AREG: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Belongs to the amphiregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cell surface; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; extracellular space

Molecular Function: epidermal growth factor receptor binding; growth factor activity; protein binding

Biological Process: cell proliferation; cell-cell signaling; COPII coating of Golgi vesicle; epidermal growth factor receptor signaling pathway; ER to Golgi vesicle-mediated transport; G-protein coupled receptor protein signaling pathway; positive regulation of cell proliferation; positive regulation of DNA replication

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Product Notes

The AREG areg (Catalog #AAA692251) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human Amphiregulin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Amphiregulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Researchers should empirically determine the suitability of the AREG areg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Amphiregulin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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