Rabbit anti-Human Amphiregulin Polyclonal Antibody | anti-AREG antibody

Rabbit Anti-Human Amphiregulin

Cat. Num. AAA692251

• Gene Names: AREG; AR; SDGF; AREGB; CRDGF
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Synonyms: Amphiregulin; Polyclonal Antibody; Rabbit Anti-Human Amphiregulin; AREG; AR; SDGF; AREGB; CRDGF; anti-AREG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Form/Format: Lyophilized
• Sequence Length: 252

• Applicable Applications for anti-AREG antibody: Western Blot (WB), Immunofluorescence (IF)
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
• Antigen: Recombinant Human Amphiregulin
• Preparation and Storage: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C.

Western Blot (WB)

(Western analysis of recombinant human Amphiregulin using a rabbit anti-human Amphiregulin polyclonal antibody.)

Immunofluorescence (IF)

(Immunofluorescence staining of human Amphiregulin in a mixture of primary human endothelial cells (HDLEC, HUVEC) and primary normal human dermal firbroblast (NHDF) with a polyclonal rabbit anti-human Amphiregulin antibody. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) [Dianova].)

Immunofluorescence (IF)

(Immunofluorescence staining of human Amphiregulin in primary normal human dermal fibroblasts (NHDF) and primary human dermal lymphatic endothelial cells (HDLEC) with a polyclonal rabbit anti-human Amphiregulin antibody. Conjugated secondary antibody: donkey anti-rabbit ALEXA Flour 488 (1:600) Dianova].)

Immunofluorescence (IF)

(Immunofluorescence staining (cryo-sections) of vasa vasorum of a human lymphatic collector. (fixed 15 min in 4% PFA) with anti-human Amphiregulin (5ug/ml) and counter staining of nuclei with Dapi. [400X] The experiment was performed by the research group of Prof. Dr. J. Wilting and Dr. K. Buttler, University Göttingen, Germany.)

Related Product Information for anti-AREG antibody

Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulins extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant human Amphiregulin is a 11.3 kDa glycoprotein consisting of 98 amino acid residues.

Product Categories/Family for anti-AREG antibody

Antibody; Polyclonal Antibody; Amphiregulin

NCBI and Uniprot Product Information

• NCBI GI #: 4502199
• NCBI GeneID: 374
• NCBI Accession #: NP_001648.1
• NCBI GenBank Nucleotide #: NM_001657.3
• UniProt Accession #: P15514; Q5U026
• Molecular Weight: 27,895 Da
• NCBI Official Full Name: amphiregulin preproprotein
• NCBI Official Synonym Full Names: amphiregulin
• NCBI Official Symbol: AREG
• NCBI Official Synonym Symbols: AR; SDGF; AREGB; CRDGF
• NCBI Protein Information: amphiregulin
• UniProt Protein Name: Amphiregulin
• Protein Family: Amphiregulin
• UniProt Gene Name: AREG
• UniProt Synonym Gene Names: CRDGF
• UniProt Entry Name: AREG_HUMAN

Uniprot Description

AREG: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Belongs to the amphiregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cell surface; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; extracellular space

Molecular Function: epidermal growth factor receptor binding; growth factor activity; protein binding

Biological Process: cell proliferation; cell-cell signaling; COPII coating of Golgi vesicle; epidermal growth factor receptor signaling pathway; ER to Golgi vesicle-mediated transport; G-protein coupled receptor protein signaling pathway; positive regulation of cell proliferation; positive regulation of DNA replication