Rabbit anti-Human Myosin IE (MYO1E) Polyclonal Antibody | anti-MYO1E antibody

Polyclonal Antibody to Myosin IE (MYO1E)

Cat. Num. AAA2006302

• Gene Names: MYO1E; FSGS6; MYO1C; HuncM-IC
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Myosin IE (MYO1E); Polyclonal Antibody; Polyclonal Antibody to Myosin IE (MYO1E); anti-MYO1E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against MYO1E. It has been selected for its ability to recognize MYO1E in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 0.95mg/ml (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGS-KRDLLLTPKC LYLIGREKVK QGPDKGLVKE VLKRKIEIER ILSVSLSTMQ DDIFILHEQE YDSLLESVFK TEFLSLLAKR YEEKTQKQLP LKFSNTLELK LKKENWGPWS AGGSRQVQFH QGFGDLAVLK PSNKVLQVSI GPGLPKNSRP TRRNTTQNTG YSSGTQNANY PVRAAPPPPG YHQNGVIRNQ YVPYPHAPGS QRSNQKSLYT SMARPPLPRQ QSTSSDRVSQ TPESLDFLKV PDQGAAGVRR QTTSRP
• Sequence Length: 1108

• Applicable Applications for anti-MYO1E antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant MYO1E (Lys781~Pro1036) expressed in E.coli.
• Cross Reactivity: Human
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2071497
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:HumanTissue))

NCBI and Uniprot Product Information

• NCBI GI #: 55956916
• NCBI GeneID: 4643
• NCBI Accession #: NP_004989.2
• NCBI GenBank Nucleotide #: NM_004998.3
• UniProt Accession #: Q12965; Q14778
• Molecular Weight: 127,062 Da
• NCBI Official Full Name: unconventional myosin-Ie
• NCBI Official Synonym Full Names: myosin IE
• NCBI Official Symbol: MYO1E
• NCBI Official Synonym Symbols: FSGS6; MYO1C; HuncM-IC
• NCBI Protein Information: unconventional myosin-Ie
• UniProt Protein Name: Unconventional myosin-Ie
• Protein Family: Unconventional myosin
• UniProt Gene Name: MYO1E
• UniProt Synonym Gene Names: MYO1C

NCBI Description

This gene encodes a member of the nonmuscle class I myosins which are a subgroup of the unconventional myosin protein family. The unconventional myosin proteins function as actin-based molecular motors. Class I myosins are characterized by a head (motor) domain, a regulatory domain and a either a short or long tail domain. Among the class I myosins, this protein is distinguished by a long tail domain that is involved in crosslinking actin filaments. This protein localizes to the cytoplasm and may be involved in intracellular movement and membrane trafficking. Mutations in this gene are the cause of focal segmental glomerulosclerosis-6. This gene has been referred to as myosin IC in the literature but is distinct from the myosin IC gene located on chromosome 17. [provided by RefSeq, Jan 2012]

Uniprot Description

MYO1E: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. Binds to membranes containing anionic phospholipids via its tail domain. Required for normal morphology of the glomerular basement membrane, normal development of foot processes by kidney podocytes and normal kidney function. In dendritic cells, may control the movement of class II-containing cytoplasmic vesicles along the actin cytoskeleton by connecting them with the actin network via ARL14EP and ARL14. Defects in MYO1E are the cause of focal segmental glomerulosclerosis type 6 (FSGS6). A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. FSGS6 is a childhood-onset disorder resulting in nephrotic syndrome, which includes massive proteinuria, hypoalbuminemia, hyperlipidemia, and edema.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: actin cytoskeleton; adherens junction; brush border; clathrin-coated endocytic vesicle; cytoplasm; cytoskeleton; extracellular exosome; intercellular junction; myosin complex

Molecular Function: actin filament binding; ATP binding; ATPase activity, coupled; calmodulin binding; microfilament motor activity; motor activity; phosphatidylinositol binding; protein binding

Biological Process: actin filament-based movement; endocytosis; glomerular basement membrane development; glomerular filtration; glomerular visceral epithelial cell development; in utero embryonic development; nitrogen compound metabolic process; platelet-derived growth factor receptor signaling pathway; post-embryonic hemopoiesis; vasculogenesis

Disease: Focal Segmental Glomerulosclerosis 6