Rabbit anti-Human AMOT Polyclonal Antibody | anti-AMOT antibody

AMOT, Phosphorylated (S1041) (AMOT, KIAA1071, Angiomotin) (AP)

Cat. Num. AAA6273323

• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AMOT; Polyclonal Antibody; Phosphorylated (S1041) (AMOT; KIAA1071; Angiomotin) (AP); Angiomotin; anti-AMOT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-AMOT antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Phospho-AMOT-S1041 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1041 of human AMOT.
• Conjugate: AP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-AMOT-S1041 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-AMOT antibody

This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Categories/Family for anti-AMOT antibody

Antibodies; KIAA; orf

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Heller, B., et al. J. Biol. Chem. 285(16):12308-12320(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Gagne, V., et al. Cell Motil. Cytoskeleton 66(9):754-768(2009). Zheng, Y., et al. Circ. Res. 105(3):260-270(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 166064029
• NCBI GeneID: 154796
• NCBI Accession #: NP_001106962.1
• NCBI GenBank Nucleotide #: NM_001113490.1
• UniProt Accession #: Q4VCS5; Q504X5; Q9HD27; Q9UPT1
• Molecular Weight: 72,540 Da
• NCBI Official Full Name: angiomotin isoform 1
• NCBI Official Synonym Full Names: angiomotin
• NCBI Official Symbol: AMOT
• NCBI Protein Information: angiomotin
• UniProt Protein Name: Angiomotin
• Protein Family: Angiomotin
• UniProt Gene Name: AMOT
• UniProt Synonym Gene Names: KIAA1071
• UniProt Entry Name: AMOT_HUMAN

NCBI Description

This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

AMOT: Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. Belongs to the angiomotin family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: signalosome; ruffle; cell surface; tight junction; endocytic vesicle; lamellipodium; cytoplasm; integral to membrane; stress fiber; actin filament; cytosol; external side of plasma membrane

Molecular Function: angiostatin binding; protein binding; receptor activity

Biological Process: intercellular junction assembly; blood vessel endothelial cell migration; positive regulation of cell size; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; gastrulation with mouth forming second; chemotaxis; regulation of cell migration; negative regulation of angiogenesis; regulation of small GTPase mediated signal transduction; positive regulation of embryonic development; positive regulation of stress fiber formation; negative regulation of vascular permeability; vasculogenesis; actin cytoskeleton organization and biogenesis; cell migration involved in gastrulation