Goat anti-Human ALS2CR2 Polyclonal Antibody | anti-STRADB antibody

ALS2CR2, CT (STE20-related Kinase Adapter Protein beta, STRAD beta, Pseudokinase ALS2CR2, Amyotrophic Lateral Sclerosis 2 Chromosomal Region Candidate Gene 2 Protein, ILP-interacting Protein, CALS-21, STRADB, ILPIP, PRO1038)

Cat. Num. AAA6000276

• Gene Names: STRADB; PAPK; ILPIP; ILPIPA; ALS2CR2; CALS-21; PRO1038
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ALS2CR2; Polyclonal Antibody; CT (STE20-related Kinase Adapter Protein beta; STRAD beta; Pseudokinase ALS2CR2; Amyotrophic Lateral Sclerosis 2 Chromosomal Region Candidate Gene 2 Protein; ILP-interacting Protein; CALS-21; STRADB; ILPIP; PRO1038); Anti -ALS2CR2; anti-STRADB antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human STRADB. Species sequence homology: canine
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-STRADB antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Western Blot: 1-3ug/ml; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3.75ug/ml
• Immunogen: Synthetic peptide corresponding to CDFPDEKDSYWEF from the C-terminal of human ALS2CR2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Heart (formalin-fixed, paraffin-embedded) stained with STRADB antibody at 3.75ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(Antibody staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Testing Data

(HEK293 overexpressing ILPIP (RC203432) and probed with the antibody (mock transfection in first lane).)

Related Product Information for anti-STRADB antibody

ALS2CR2 potentiates the antiapoptotic activity of XIAP by enhancing XIAP-mediated activation of JNK1 (MAPK8) and other JNK family members, but not by modulating XIAP-mediated caspase inhibition. Expression of a catalytically inactive TAK1 (MAP3K7) mutant blocks the XIAP/ALS2CR2 activation of JNK1. In vivo coprecipitation experiments show that both ALS2CR2 and XIAP interact with TAK1 and TRAF6. It has been proposed that XIAP-mediated protection from apoptosis utilizes both a JNK1 activation pathway that involves ALS2CR2 and a caspase inhibition pathway that is independent of ALS2CR2.

Product Categories/Family for anti-STRADB antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 12862480
• NCBI GeneID: 55437
• UniProt Accession #: Q9C0K7; Q5BKY7; Q9P1L0
• Molecular Weight: 47,026 Da
• NCBI Official Full Name: amyotrophic lateral sclerosis 2
• NCBI Official Synonym Full Names: STE20-related kinase adaptor beta
• NCBI Official Symbol: STRADB
• NCBI Official Synonym Symbols: PAPK; ILPIP; ILPIPA; ALS2CR2; CALS-21; PRO1038
• NCBI Protein Information: STE20-related kinase adapter protein beta; STRAD beta; pseudokinase ALS2CR2; ILP-interacting protein ILPIPA; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2; amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein
• UniProt Protein Name: STE20-related kinase adapter protein beta
• Protein Family: STE20-related kinase adapter protein
• UniProt Gene Name: STRADB
• UniProt Synonym Gene Names: ALS2CR2; ILPIP; STRAD beta
• UniProt Entry Name: STRAB_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Uniprot Description

STLK6: Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, STE; Motility/polarity/chemotaxis; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; STE group; STE20 family; STLK subfamily

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of protein kinase activity; insulin receptor signaling pathway; cell morphogenesis; JNK cascade; mitotic cell cycle; protein export from nucleus; cell cycle arrest; protein amino acid phosphorylation