Mouse anti-Human Requiem / DPF2 Monoclonal Antibody | anti-DPF2 antibody

Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human Requiem / DPF2

Cat. Num. AAA245637

• Gene Names: DPF2; REQ; UBID4; ubi-d4
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: Requiem / DPF2; Monoclonal Antibody; Mouse Monoclonal [clone 2F6] (IgG2a; k) to Human Requiem / DPF2; Anti-Requiem / DPF2 Antibody (clone 2F6) IHC-plus; DPF2; BAF45D; REQ; UBID4; Zinc finger protein ubi-d4; BRG1-associated factor 45D; Ubi-d4; Protein requiem; Requiem; Human Requiem; anti-DPF2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2F6
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.43 mg/ml (varies by lot)
• Sequence Length: 391

• Applicable Applications for anti-DPF2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (2.5 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control LNCaP. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells.
• Target Species: Human
• Immunogen Description: DPF2 (AAH14889, 56 a.a. ~ 155 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: Requiem / DPF2 antibody was raised against dPF2 (AAH14889, 56 a.a. ~ 155 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-DPF2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-DPF2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to DPF2 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(Western blot of DPF2 expression in transfected 293T cell line by DPF2 monoclonal antibody clone 2F6.)

Western Blot (WB)

(DPF2 monoclonal antibody clone 2F6 Western blot of DPF2 expression in LNCaP.)

NCBI and Uniprot Product Information

• NCBI GI #: 5454004
• NCBI GeneID: 5977
• NCBI Accession #: NP_006259.1
• NCBI GenBank Nucleotide #: NM_006268.4
• UniProt Accession #: Q92785; A8K7C9; B4DT58
• Molecular Weight: 23,599 Da
• NCBI Official Full Name: zinc finger protein ubi-d4
• NCBI Official Synonym Full Names: D4, zinc and double PHD fingers family 2
• NCBI Official Symbol: DPF2
• NCBI Official Synonym Symbols: REQ; UBID4; ubi-d4
• NCBI Protein Information: zinc finger protein ubi-d4; BAF45D; protein requiem; BRG1-associated factor 45D; apoptosis response zinc finger protein; requiem, apoptosis response zinc finger
• UniProt Protein Name: Zinc finger protein ubi-d4
• Protein Family: Zinc finger protein
• UniProt Gene Name: DPF2
• UniProt Synonym Gene Names: BAF45D; REQ; UBID4; BAF45D
• UniProt Entry Name: REQU_HUMAN

NCBI Description

The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors. [provided by RefSeq, Jul 2008]

Uniprot Description

requiem: May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells. Belongs to the requiem/DPF family.

Protein type: Apoptosis; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; centrosome; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm

Molecular Function: zinc ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis