Rabbit anti-Human ALPP Polyclonal Antibody | anti-ALPP antibody

ALPP (Alkaline Phosphatase, Placental Type, Alkaline Phosphatase Regan Isozyme, Placental Alkaline Phosphatase 1, PLAP-1, PLAP) (Biotin)

Cat. Num. AAA6369673

• Gene Names: ALPP; ALP; PALP; PLAP; PLAP-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ALPP; Polyclonal Antibody; ALPP (Alkaline Phosphatase; Placental Type; Alkaline Phosphatase Regan Isozyme; Placental Alkaline Phosphatase 1; PLAP-1; PLAP) (Biotin); anti-ALPP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALPP.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ALPP antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human ALPP, aa1-535 (NP_001623.3).
• Immunogen Sequence: MLGPCMLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTDAAHPGRSVVPALLPLLAGTLLLLETATAP
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of ALPP expression in transfected 293T cell line by ALPP polyclonal antibody. Lane 1: ALPP transfected lysate (58kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-ALPP antibody

Alkaline phosphatases are dimeric metalloenzymes which catalyses the hydrolysis of phosphomonoesters with release of inorganic phosphate and alcohol. In humans, three (placental, PLAP; germ cell, GCAP; and intestinal, IAP) out of the four alkaline phosphatases isozymes are tissue-specific. These three are 90-98% homologous and their genes are clustered on chromosome 2 while the fourth isoenzyme is tissue non-specific and is located on chromosome 1. The placental isoenzyme (PLAP), the most stable of the isoenzymes has attracted much interest because it is structurally related to some of the tumor-associated alkaline phosphatases. The crystal structure of human PLAP revealed three additional domains compared to the bacterial enzyme which correspond to an interfacial crown domain, an N-terminal alpha-helix and a peripheral calcium-binding domain. The enzyme is heat labile and is enzymatically inefficient, with a lower catalytic constant (kcat 460 s-1). It plays a role in transfer of maternal IgG to the fetus. More recently, it was reported that PLAP stimulates DNA synthesis and cell proliferation in fibroblasts in concert with insulin, zinc and calcium ions.

Product Categories/Family for anti-ALPP antibody

Antibodies; Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 94721246
• NCBI GeneID: 250
• NCBI Accession #: NP_001623.3
• NCBI GenBank Nucleotide #: NM_001632.3
• UniProt Accession #: P05187; P05188; P06861; Q53S78; Q96DB7
• Molecular Weight: 57,954 Da
• NCBI Official Full Name: alkaline phosphatase, placental type preproprotein
• NCBI Official Synonym Full Names: alkaline phosphatase, placental
• NCBI Official Symbol: ALPP
• NCBI Official Synonym Symbols: ALP; PALP; PLAP; PLAP-1
• NCBI Protein Information: alkaline phosphatase, placental type; Regan isozyme; glycerophosphatase; alkaline phosphomonoesterase; placental alkaline phosphatase 1; alkaline phosphatase Regan isozyme
• UniProt Protein Name: Alkaline phosphatase, placental type
• Protein Family: Alkaline phosphatase
• UniProt Gene Name: ALPP
• UniProt Synonym Gene Names: PLAP; PLAP-1
• UniProt Entry Name: PPB1_HUMAN

NCBI Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

Protein type: Cell surface; Membrane protein, integral; Cofactor and Vitamin Metabolism - folate biosynthesis; Phosphatase (non-protein); Motility/polarity/chemotaxis; Membrane protein, GPI anchor; EC 3.1.3.1

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: cell surface; plasma membrane; integral to membrane

Molecular Function: zinc ion binding; alkaline phosphatase activity; magnesium ion binding

Biological Process: dephosphorylation