Mouse anti-Human CD44 Monoclonal Antibody | anti-CD44 antibody

anti-human CD44-Biotin

Cat. Num. AAA666471

• Gene Names: CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Synonyms: CD44; Monoclonal Antibody; anti-human CD44-Biotin; Monoclonal anti-human CD44; anti-CD44 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: BU75
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 43

• Applicable Applications for anti-CD44 antibody: Flow Cytometry (FC/FACS), Western Blot (WB)
• Performance: Five x 105 cultured Molt-4 human tumor cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD44 antibody at a concentration of 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.75 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.
• Conjugation: Biotin
• Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2u sterile filtered and vialed under aseptic conditions.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
• Immunogen: Human peripheral myeloma cells
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD44 antibody

Information: Human CD44 molecules appear to serve as lymphocyte adhesion molecules with numerous functions in extracellular matrix binding, cell migration, hemopoisis, and lymphocyte homing. Antibody BU75 recognizes epitope 2 of the CD44 molecule which interacts with hyaluronate (HA) mediating binding of lymphocytes to high endothelial venules. Antibody BU75 blocks binding of HA to CD44.

References

B.F. Hynes, et al, (1993) Adv Immunol 54: 271-335. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1713-1742. H.X. Liao, et al, (1995) J Immunol 155: 3938-3945.

NCBI and Uniprot Product Information

• NCBI GI #: 685122
• NCBI GeneID: 960
• Molecular Weight: 15,635 Da
• NCBI Official Full Name: CD44, partial
• NCBI Official Synonym Full Names: CD44 molecule (Indian blood group)
• NCBI Official Symbol: CD44
• NCBI Official Synonym Symbols: IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
• NCBI Protein Information: CD44 antigen; epican; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; heparan sulfate proteoglycan; cell surface glycoprotein CD44; extracellular matrix receptor III; chondroitin sulfate proteoglycan 8; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; homing function and Indian blood group system
• UniProt Protein Name: CD44 antigen
• Protein Family: CD44 antigen
• UniProt Gene Name: CD44
• UniProt Synonym Gene Names: LHR; MDU2; MDU3; MIC4; ECMR-III; PGP-1; PGP-I
• UniProt Entry Name: CD44_HUMAN

NCBI Description

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

CD44: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. 19 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Receptor, misc.

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: Golgi apparatus; focal adhesion; cell surface; basolateral plasma membrane; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: collagen binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; hyaluronic acid binding; hyalurononglucosaminidase activity

Biological Process: extracellular matrix organization and biogenesis; positive regulation of heterotypic cell-cell adhesion; Wnt receptor signaling pathway; glycosaminoglycan metabolic process; cytokine and chemokine mediated signaling pathway; cell-matrix adhesion; pathogenesis; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; hyaluronan catabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; extracellular matrix disassembly; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell adhesion; ureteric bud branching; cartilage development; carbohydrate metabolic process; blood coagulation; healing during inflammatory response; hyaluronan metabolic process; leukocyte migration; negative regulation of apoptosis

Disease: Blood Group, Indian System