Epidermal Growth Factor (EGF) Recombinant Protein | EGF recombinant protein

Recombinant Epidermal Growth Factor (EGF)

Cat. Num. AAA2010442

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Epidermal Growth Factor (EGF); Recombinant Epidermal Growth Factor (EGF); EGF recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-APFLIF SHGNSIFRID LEGTNHEQLV ADAGISVLMD FHYNEERIYW VDLERQLLQR VFLNGTRQEK VCNLEKNVSG MAINWINEEL IWSNQQEGTI TVTDMKGNNS RVLLSALKYP ANVAVDPVER LMFWSSVVAG SLHRADVTGV EVRLLLETSE EIAAVSLDVL DKRLFWIQYN REGGSSRICS CDYDGGSVHF SKHLTQHNVF AMSLFGDHIF YSTWKKKTIW VANKH
• Sequence Length: 1214

• Applicable Applications for EGF recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Sus scrofa; Porcine (Pig)
• Expression System: Prokaryotic expression
• Residues: Ala45~His275 (Accession # Q00968) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 47522862
• NCBI GeneID: 397083
• NCBI Accession #: NP_999185.1
• NCBI GenBank Nucleotide #: NM_214020.1
• UniProt Accession #: Q00968; O62759; Q9BDQ0
• Molecular Weight: 32.1kDa
• NCBI Official Full Name: pro-epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Protein Information: pro-epidermal growth factor
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: Pro-epidermal growth factor
• UniProt Gene Name: EGF
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_PIG

Uniprot Description

Function: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6

By similarity.

Subunit structure: Interacts with EGFR and promotes EGFR dimerization. Interacts with RHBDF1; may retain EGF in the endoplasmic reticulum and regulates its degradation through the endoplasmic reticulum-associated degradation (ERAD)

By similarity. Interacts with RHBDF2

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein.

Sequence similarities: Contains 9 EGF-like domains.Contains 9 LDL-receptor class B repeats.