Western Blot (WB)
(Anti-NAPA rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDaObserved band size:36 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human Alpha SNAP Polyclonal Antibody | anti-NAPA antibody
Anti-Alpha SNAP Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
ICC/IF: 1:300-1:10,000
IP: 0.5-2uL/mg of lysate
Western Blot (WB)
(Anti-NAPA rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDaObserved band size:36 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-NAPA rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:33 kDaObserved band size:36 kDa(We are unsure as to the identity of these extra bands.))
Immunofluorescence (IF)
(Immunofluorescence staining of NAPA in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NAPA polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of NAPA in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human NAPA polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Immunoprecipitation (IP)
(NAPA was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-NAPA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NAPA rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 36 kDa)
Immunoprecipitation (IP)
(NAPA was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate4 uL anti-NAPA rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NAPA rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size: 36 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the soluble NSF attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the completion of membrane fusion by mediating the interaction of N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated and membrane-associated SNAP receptor (SNARE) complex, and stimulating the ATPase activity of NSF. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jun 2011]
Uniprot Description
SNAP-alpha: Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. Belongs to the SNAP family.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: SNARE complex; membrane; vacuolar membrane; cytosol
Molecular Function: protein binding; syntaxin binding; soluble NSF attachment protein activity
Biological Process: neuron differentiation; synaptic transmission, glutamatergic; intracellular protein transport; intra-Golgi vesicle-mediated transport; apical protein localization; brain development; post-Golgi vesicle-mediated transport
Research Articles on NAPA
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Product Notes
The NAPA napa (Catalog #AAA8107483) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Alpha SNAP Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha SNAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 ICC/IF: 1:300-1:10,000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the NAPA napa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha SNAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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