Western Blot (WB)
(Western blot analysis SPT4H using 293 whole cell lysates)
Rabbit anti-Human, Mouse SPT4H Polyclonal Antibody | anti-SPT4H antibody
SPT4H Antibody
Western Blot (WB)
(Western blot analysis SPT4H using 293 whole cell lysates)
Western Blot (WB)
(Western blot analysis SPT4H using 293 whole cell lysates)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the small subunit of DRB (5,6-dichloro-1-beta-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. The encoded protein is localized to the nucleus and interacts with the large subunit (SUPT5H) to form the DSIF complex. Related pseudogenes have been identified on chromosomes 2 and 12. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
Uniprot Description
SUPT4H1: Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV- 1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. Belongs to the SPT4 family.
Chromosomal Location of Human Ortholog: 17q22
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein binding; protein heterodimerization activity; transcription factor activity
Biological Process: chromatin remodeling; negative regulation of RNA elongation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of RNA elongation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of viral transcription; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Research Articles on SPT4H
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Product Notes
The SPT4H supt4h1 (Catalog #AAA9418904) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPT4H Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPT4H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1:1000-3000. Researchers should empirically determine the suitability of the SPT4H supt4h1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPT4H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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