Mouse CD36 Monoclonal Antibody | anti-CD36 antibody

CD36 (Platelet & Microvessel Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439681

• Gene Names: CD36; FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
• Reactivity: Human; Others not known.
• Applications: Flow Cytometry, Immunofluorescence
• Synonyms: CD36; Monoclonal Antibody; CD36 (Platelet & Microvessel Marker) Mouse Monoclonal Antibody; Adipocyte membrane protein; CHDS7; Collagen receptor; platelet; fatty acid translocase (FAT); Fatty acid transport protein; glycoprotein iiib (GP IIIb); PAS IV; PAS-4; pas4 protein; platelet collagen receptor; Platelet glycoprotein 4; scarb3; Scavenger receptor class B member 3; thrombospondin receptor; anti-CD36 antibody

• Host: Mouse
• Reactivity: Human; Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 1E8
• Specificity: Recognizes a protein of 80kDa-90kDa, identified as CD36. It is expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P. falciparum malaria-infected erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL) and recognition of apoptotic polymorphonuclear leukocytes (PMN). CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria. Note that 1-4% of Japanese and East Asia population lack CD36.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 472

• Applicable Applications for anti-CD36 antibody: Functional Studies, Flow Cytometry (FC), Immunofluorescence (IF)
• Application Notes: Functional Studies (Order Ab without Azide); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Optimal dilution for a specific application should be determined.
• Cellular Localization: Cell Surface
• Immunogen: Human CD36 from platelets
• Human Chromosome Location: 7q11.2
• Positive Control: HEL or U937 cells. Platelets, monocytes, macrophages, microvascular endothelial cells in a tonsil.
• Preparation and Storage: Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified CD36 Mouse Monoclonal Antibody (1E8).Confirmation of Purity and Integrity of Antibody.)

References

Stomski FC; Gani JS; Bates RC; Burns GF. Adhesion to thrombospondin by human embryonic fibroblasts is mediated by multiple receptors and includes a role for glycoprotein 88 (CD36). Experimental Cell Research, 1992, 198(1):85-92. Biggs BA; Gooze L; Wycherley K; Wilkinson D; Boyd AW; Forsyth KP; Edelman L; Brown GV; Leech JH. Knob-independent cytoadherence of Plasmodium falciparum to the leukocyte differentiation antigen CD36. Journal of Experimental Medicine, 1990, 171(6):1883-92

NCBI and Uniprot Product Information

• NCBI GI #: 48375178
• NCBI GeneID: 948
• NCBI Accession #: NP_000063.2
• NCBI GenBank Nucleotide #: NM_000072.3
• UniProt Accession #: P16671; Q13966; Q16093; Q8TCV7; Q9BPZ8; Q9BQC2; Q9BZM8; Q9BZN3; D9IX66; D9IX67; D9IX68; D9IX69
• Molecular Weight: 80-90kDa
• NCBI Official Full Name: platelet glycoprotein 4 isoform 1
• NCBI Official Synonym Full Names: CD36 molecule
• NCBI Official Symbol: CD36
• NCBI Official Synonym Symbols: FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
• NCBI Protein Information: platelet glycoprotein 4
• UniProt Protein Name: Platelet glycoprotein 4
• Protein Family: Platelet glycoprotein
• UniProt Gene Name: CD36
• UniProt Synonym Gene Names: GP3B; GP4; FAT; GPIIIB; GPIV
• UniProt Entry Name: CD36_HUMAN

NCBI Description

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

CD36: Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7). Belongs to the CD36 family.

Protein type: Membrane protein, multi-pass; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q11.2

Cellular Component: brush border membrane; cell surface; extracellular space; Golgi apparatus; integral to plasma membrane; lipid raft; membrane; phagocytic vesicle; plasma membrane; platelet alpha granule membrane

Molecular Function: lipid binding; low-density lipoprotein binding; low-density lipoprotein receptor activity; protein binding; transforming growth factor beta binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; blood coagulation; cGMP-mediated signaling; cholesterol absorption; cholesterol transport; elevation of cytosolic calcium ion concentration; interleukin-1 beta secretion; intestinal absorption; lipoprotein transport; MyD88-dependent toll-like receptor signaling pathway; nitric oxide mediated signal transduction; platelet degranulation; positive regulation of cell-matrix adhesion; receptor internalization; receptor-mediated endocytosis; response to lipid; sensory perception of taste; sequestering of lipid; triacylglycerol transport

Disease: Coronary Heart Disease, Susceptibility To, 7; Malaria, Susceptibility To; Platelet Glycoprotein Iv Deficiency