Western Blot (WB)
(ARHGEF5 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ARHGEF5 antibody detected the ARHGEF5 protein (arrow).)
Rabbit anti-Human ARHGEF5 Polyclonal Antibody | anti-ARHGEF5 antibody
ARHGEF5, CT (ARHGEF5, TIM, Rho guanine nucleotide exchange factor 5, Ephexin-3, Guanine nucleotide regulatory protein TIM, Oncogene TIM, Transforming immortalized mammary oncogene, p60 TIM) (PE)
Gene Names
ARHGEF5; P60; TIM; GEF5; TIM1
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ARHGEF5; Polyclonal Antibody; CT (ARHGEF5; TIM; Rho guanine nucleotide exchange factor 5; Ephexin-3; Guanine nucleotide regulatory protein TIM; Oncogene TIM; Transforming immortalized mammary oncogene; p60 TIM) (PE); p60 TIM; anti-ARHGEF5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-ARHGEF5 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
ARHGEF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1502-1530 amino acids from the C-terminal region of human ARHGEF5.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(ARHGEF5 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ARHGEF5 antibody detected the ARHGEF5 protein (arrow).)
Western Blot (WB)
(ARHGEF5 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ARHGEF5 antibody detected the ARHGEF5 protein (arrow).)
Related Product Information for anti-ARHGEF5 antibody
Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.
Product Categories/Family for anti-ARHGEF5 antibody
References
Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Wang, Z., et al. J. Biol. Chem. 284(42):28599-28606(2009). Lin, Y.M., et al. Mol. Carcinog. 47(12):925-933(2008). Zhang, Y., et al. Mol. Cell Proteomics 4(9):1240-1250(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
176,799 Da
NCBI Official Full Name
rho guanine nucleotide exchange factor 5
NCBI Official Synonym Full Names
Rho guanine nucleotide exchange factor (GEF) 5
NCBI Official Symbol
ARHGEF5
NCBI Official Synonym Symbols
P60; TIM; GEF5; TIM1
NCBI Protein Information
rho guanine nucleotide exchange factor 5; p60 TIM; ephexin-3; oncogene TIM; guanine nucleotide regulatory protein TIM; transforming immortalized mammary oncogene
UniProt Protein Name
Rho guanine nucleotide exchange factor 5
Protein Family
UniProt Gene Name
ARHGEF5
UniProt Synonym Gene Names
TIM
UniProt Entry Name
ARHG5_HUMAN
NCBI Description
Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008]
Uniprot Description
ARHGEF5: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: GEFs; Oncoprotein; Motility/polarity/chemotaxis; GEFs, Rac/Rho
Chromosomal Location of Human Ortholog: 7q35
Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; GTP binding
Biological Process: myeloid dendritic cell chemotaxis; actin cytoskeleton organization and biogenesis
Research Articles on ARHGEF5
Similar Products
Product Notes
The ARHGEF5 arhgef5 (Catalog #AAA6275038) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARHGEF5, CT (ARHGEF5, TIM, Rho guanine nucleotide exchange factor 5, Ephexin-3, Guanine nucleotide regulatory protein TIM, Oncogene TIM, Transforming immortalized mammary oncogene, p60 TIM) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGEF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ARHGEF5 arhgef5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGEF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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