Goat anti-Human ALOX5AP Polyclonal Antibody | anti-ALOX5AP antibody

ALOX5AP (Arachidonate 5-lipoxygenase-activating protein, FLAP, MK-886-binding protein)

Cat. Num. AAA621072

• Gene Names: ALOX5AP; FLAP
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ALOX5AP; Polyclonal Antibody; ALOX5AP (Arachidonate 5-lipoxygenase-activating protein; FLAP; MK-886-binding protein); Anti -ALOX5AP (Arachidonate 5-lipoxygenase-activating protein; anti-ALOX5AP antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes ALOX5AP.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA., 0.02% sodium azide.

• Applicable Applications for anti-ALOX5AP antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blot.; Dilution: ELISA: 1:32000; Western Blot: P1ug/ml. ~48kD band in human brain and U937 lysates. This band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Synthetic peptide corresponding to the C-terminus of ALOX5AP.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ALOX5AP antibody

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Product Categories/Family for anti-ALOX5AP antibody

Antibodies; Abs to Leukotrienes

NCBI and Uniprot Product Information

• NCBI GI #: 15718675
• NCBI GeneID: 241
• NCBI Accession #: NP_001620.2
• NCBI GenBank Nucleotide #: NM_001629.3
• UniProt Accession #: P20292; Q5VV04
• Molecular Weight: 18,157 Da
• NCBI Official Full Name: arachidonate 5-lipoxygenase-activating protein isoform 1
• NCBI Official Synonym Full Names: arachidonate 5-lipoxygenase-activating protein
• NCBI Official Symbol: ALOX5AP
• NCBI Official Synonym Symbols: FLAP
• NCBI Protein Information: arachidonate 5-lipoxygenase-activating protein; OTTHUMP00000018204; MK-886-binding protein
• UniProt Protein Name: Arachidonate 5-lipoxygenase-activating protein
• Protein Family: Arachidonate 5-lipoxygenase-activating protein
• UniProt Gene Name: ALOX5AP
• UniProt Synonym Gene Names: FLAP
• UniProt Entry Name: AL5AP_HUMAN

NCBI Description

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Uniprot Description

ALOX5AP: Required for leukotriene biosynthesis by ALOX5 (5- lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. Belongs to the MAPEG family.

Protein type: Endoplasmic reticulum; Nuclear envelope; Membrane protein, integral; Activator; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; membrane; endoplasmic reticulum; integral to membrane; nuclear envelope

Molecular Function: arachidonic acid binding; protein binding; glutathione transferase activity; leukotriene-C4 synthase activity; enzyme activator activity; protein N-terminus binding; glutathione peroxidase activity

Biological Process: positive regulation of catalytic activity; leukotriene biosynthetic process; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Stroke, Ischemic