Rabbit ALOX5 Polyclonal Antibody | anti-ALOX5 antibody

ALOX5 antibody

Cat. Num. AAA9409656

• Gene Names: ALOX5; 5-LO; 5LPG; LOG5; 5-LOX
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: ALOX5; Polyclonal Antibody; ALOX5 antibody; 5-LO; 5-LOX; 5LPG; LOG5; MGC163204; anti-ALOX5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total ALOX5 antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 674

• Applicable Applications for anti-ALOX5 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500 - 1:2000
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human ALOX5.
• Target Name: ALOX5
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of HepG2 cell lines, using ALOX5 antibody.)

Related Product Information for anti-ALOX5 antibody

5-Lipoxygenase (5-LO, ALOX5) is an important catalytic enzyme responsible for the biosynthesis of leukotriene LTA4 from arachidonic acid (1,2). Leukotriene synthesis also requires 5-lipoxygenase-activating protein (FLAP, ALOX5AP), a nuclear membrane-bound protein that binds arachidonic acid and is thought to activate 5-LO. A number of related leukotrienes (i.e. B4, C4, D4) are derived from LTA4 and together these lipid mediators function in immune reaction regulation. 5-LO is primarily expressed in polymorphonuclear leukocytes, peripheral blood monocytes, macrophages, and mast cells (1,3). Overexpression of 5-LO protein is seen in certain cancer cells and is associated with poor identification (1,4). Depending upon the cell type, 5-LO is localized to either the cytosol or the nucleus of quiescent cells (5). Following stimulation, 5-LO translocates to the nucleus and associates with FLAP to catalyze LTA4 synthesis (2,3). Phosphorylation of specific residues can regulate 5-LO enzymatic activity. Phosphorylation of 5-LO at Ser523 by PKA family kinases inhibits oxygenase activity (6,7) while MAPKAP2 and ERK family kinase phosphorylation at Ser271 and Ser663 stimulates 5-LO enzymatic activity in vivo (8,9).

Product Categories/Family for anti-ALOX5 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4502057
• NCBI GeneID: 240
• NCBI Accession #: NP_000689.1
• NCBI GenBank Nucleotide #: NM_000698.3
• UniProt Accession #: P09917; Q5JQ14; B7ZLS0; E5FPY5; E5FPY7; E5FPY8
• Molecular Weight: 56,291 Da
• NCBI Official Full Name: arachidonate 5-lipoxygenase isoform 1
• NCBI Official Synonym Full Names: arachidonate 5-lipoxygenase
• NCBI Official Symbol: ALOX5
• NCBI Official Synonym Symbols: 5-LO; 5LPG; LOG5; 5-LOX
• NCBI Protein Information: arachidonate 5-lipoxygenase
• UniProt Protein Name: Arachidonate 5-lipoxygenase
• Protein Family: Arachidonate 5-lipoxygenase
• UniProt Gene Name: ALOX5
• UniProt Synonym Gene Names: LOG5; 5-LO; 5-lipoxygenase
• UniProt Entry Name: LOX5_HUMAN

NCBI Description

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.

Protein type: Nuclear envelope; Lipid Metabolism - linoleic acid; EC 1.13.11.34; Oxidoreductase; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol

Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity

Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Asthma, Susceptibility To