Human Arachidonate 5-lipoxygenase ELISA Kit | ALOX5 elisa kit

Human Arachidonate 5-lipoxygenase ELISA Kit

Cat. Num. AAA288878

• Gene Names: ALOX5; 5-LO; 5LPG; LOG5; 5-LOX
• Reactivity: Human
• Synonyms: Arachidonate 5-lipoxygenase; Human Arachidonate 5-lipoxygenase ELISA Kit; 5-LO; 5-lipoxygenase; ALOX5; LOG5; 1.13.11.34; ALOX5 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human 5-lipoxygenase
• Sequence Length: 674

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: <0.078 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.6%
• Inter-assay Precision: Inter-Assay CV: <=7.5%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALOX5 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502057
• NCBI GeneID: 240
• NCBI Accession #: NP_000689.1
• NCBI GenBank Nucleotide #: NM_000698.3
• UniProt Accession #: P09917; Q5JQ14; B7ZLS0; E5FPY5; E5FPY7; E5FPY8
• Molecular Weight: 56,291 Da
• NCBI Official Full Name: arachidonate 5-lipoxygenase isoform 1
• NCBI Official Synonym Full Names: arachidonate 5-lipoxygenase
• NCBI Official Symbol: ALOX5
• NCBI Official Synonym Symbols: 5-LO; 5LPG; LOG5; 5-LOX
• NCBI Protein Information: arachidonate 5-lipoxygenase
• UniProt Protein Name: Arachidonate 5-lipoxygenase
• Protein Family: Arachidonate 5-lipoxygenase
• UniProt Gene Name: ALOX5
• UniProt Synonym Gene Names: LOG5; 5-LO; 5-lipoxygenase
• UniProt Entry Name: LOX5_HUMAN

NCBI Description

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.

Protein type: Oxidoreductase; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid; EC 1.13.11.34; Nuclear envelope

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cytosol; dendrite; extracellular space; nuclear envelope; nuclear envelope lumen; nuclear matrix; nuclear membrane; sarcolemma

Molecular Function: arachidonate 5-lipoxygenase activity; iron ion binding; protein binding

Biological Process: acute inflammatory response; arachidonic acid metabolic process; leukotriene biosynthetic process; leukotriene metabolic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; positive regulation of vasoconstriction; response to hyperoxia; response to nutrient; sensory perception of pain

Disease: Asthma, Susceptibility To