Western Blot (WB)
(Western Blot analysis of ALKBH5 in A549 and Hela cell lysates in order using ALKBH5 Rabbit pAb at dilution 1/600)
Rabbit ALKBH5 Polyclonal Antibody | anti-ALKBH5 antibody
ALKBH5 Rabbit pAb
Gene Names
ALKBH5; ABH5; OFOXD; OFOXD1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purified
Synonyms
ALKBH5; Polyclonal Antibody; ALKBH5 Rabbit pAb; alkB homolog 5; RNA demethylase; ABH5; OFOXD; OFOXD1; anti-ALKBH5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.66mg/ml (varies by lot)
Applicable Applications for anti-ALKBH5 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Synthetic peptide of human ALKBH5
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Western Blot analysis of ALKBH5 in A549 and Hela cell lysates in order using ALKBH5 Rabbit pAb at dilution 1/600)
Western Blot (WB)
(Western Blot analysis of ALKBH5 in A549 and Hela cell lysates in order using ALKBH5 Rabbit pAb at dilution 1/600)
Related Product Information for anti-ALKBH5 antibody
Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N6-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,049 Da
NCBI Official Full Name
RNA demethylase ALKBH5
NCBI Official Synonym Full Names
alkB homolog 5, RNA demethylase
NCBI Official Symbol
ALKBH5
NCBI Official Synonym Symbols
ABH5; OFOXD; OFOXD1
NCBI Protein Information
RNA demethylase ALKBH5
UniProt Protein Name
RNA demethylase ALKBH5
Protein Family
UniProt Gene Name
ALKBH5
UniProt Synonym Gene Names
ABH5; OFOXD1
Uniprot Description
ALKBH5: a protein contaning a 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily domain. May be involved in DNA alkylation damage repair
Protein type: DNA repair, damage; EC 1.14.11.-; Membrane protein, integral; Oxidoreductase; RNA-binding
Chromosomal Location of Human Ortholog: 17p11.2|17p11.2
Cellular Component: cytosol; Golgi apparatus; nuclear speck; nucleoplasm; nucleus
Molecular Function: metal ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; RNA binding
Biological Process: cell differentiation; DNA dealkylation involved in DNA repair; mRNA export from nucleus; mRNA processing; response to hypoxia; spermatogenesis
Research Articles on ALKBH5
Similar Products
Product Notes
The ALKBH5 alkbh5 (Catalog #AAA4759869) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALKBH5 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ALKBH5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ALKBH5 alkbh5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALKBH5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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