Western Blot (WB)
(Western BlotPositive WB detected in:Mouse spleen tissue,Mouse lung tissue,Mouse kidney tissueAll lanes: ALKBH4 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 34,5 kDaObserved band size: 34 kDa)
Rabbit anti-Human, Mouse ALKBH4 Polyclonal Antibody | anti-ALKBH4 antibody
ALKBH4 Antibody
Gene Names
ALKBH4; ABH4
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
ALKBH4; Polyclonal Antibody; ALKBH4 Antibody; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4; Alkylated DNA repair protein alkB homolog 4; ABH4; anti-ALKBH4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
302
Applicable Applications for anti-ALKBH4 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1: 2000-1: 5000
IF: 1: 50-1: 200
IF: 1: 50-1: 200
Immunogen
Recombinant human Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4 protein (1-115AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-244888 / sc-244889
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western BlotPositive WB detected in:Mouse spleen tissue,Mouse lung tissue,Mouse kidney tissueAll lanes: ALKBH4 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 34,5 kDaObserved band size: 34 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in:Mouse spleen tissue,Mouse lung tissue,Mouse kidney tissueAll lanes: ALKBH4 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 34,5 kDaObserved band size: 34 kDa)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS7051441 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS7051441 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-ALKBH4 antibody
Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. May be involved in transcription regulation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
4,556 Da
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase alkB homolog 4
NCBI Official Synonym Full Names
alkB homolog 4, lysine demethylase
NCBI Official Symbol
ALKBH4
NCBI Official Synonym Symbols
ABH4
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase alkB homolog 4
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4
Protein Family
UniProt Gene Name
ALKBH4
UniProt Synonym Gene Names
ABH4
UniProt Entry Name
ALKB4_HUMAN
Uniprot Description
ALKBH4: Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron. Belongs to the alkB family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.-
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: midbody
Molecular Function: actin binding; demethylase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; protein binding
Biological Process: actomyosin structure organization and biogenesis; protein amino acid demethylation
Research Articles on ALKBH4
Similar Products
Product Notes
The ALKBH4 alkbh4 (Catalog #AAA7051441) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALKBH4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ALKBH4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1: 2000-1: 5000 IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the ALKBH4 alkbh4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALKBH4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
