SDS-Page
(Recombinant ALKBH4 protein gel 12.5% SDS-PAGE Coomassie staining MW: 37.4 kDa Purity: >85%)
ALKBH4 recombinant protein
Recombinant ALKBH4 protein
Gene Names
ALKBH4; ABH4
Synonyms
ALKBH4; Recombinant ALKBH4 protein; Alpha-Ketoglutarate-Dependent Dioxygenase AlkB Homolog 4; ALKBH4 recombinant protein
Host
E Coli
Form/Format
Recombinant ALKBH4 protein is supplied in 25mM Tris-HCl pH8.0, 300mM NaCl, 10% glycerol, and 0.5mM TCEP
Protein Species
Human
Tag
His-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant ALKBH4 protein gel 12.5% SDS-PAGE Coomassie staining MW: 37.4 kDa Purity: >85%)
SDS-Page
(Recombinant ALKBH4 protein gel 12.5% SDS-PAGE Coomassie staining MW: 37.4 kDa Purity: >85%)
Related Product Information for ALKBH4 recombinant protein
Short Description: Full-length ALKBH4 protein (accession number NP_060091.1) was expressed in E Coli with an N-terminal 6xHis-tag. The molecular weight of ALKBH4 is 37.4 kDa.
Background: ALKBH4 (Alpha-Ketoglutarate-Dependent Dioxygenase AlkB Homolog 4) is a member of ALKBH protein family. It's a dioxygenase that mediates demethylation of actin monomethylated at Lys-84 (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. ALKBH4 may also be involved in transcription regulation.
Background: ALKBH4 (Alpha-Ketoglutarate-Dependent Dioxygenase AlkB Homolog 4) is a member of ALKBH protein family. It's a dioxygenase that mediates demethylation of actin monomethylated at Lys-84 (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. ALKBH4 may also be involved in transcription regulation.
Product Categories/Family for ALKBH4 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
4,556 Da
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase alkB homolog 4
NCBI Official Synonym Full Names
alkB homolog 4, lysine demethylase
NCBI Official Symbol
ALKBH4
NCBI Official Synonym Symbols
ABH4
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase alkB homolog 4
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4
Protein Family
UniProt Gene Name
ALKBH4
UniProt Synonym Gene Names
ABH4
UniProt Entry Name
ALKB4_HUMAN
Uniprot Description
ALKBH4: Probable dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron. Belongs to the alkB family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.-
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: cytoplasm; midbody; nucleus
Molecular Function: metal ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; actin binding; demethylase activity
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; actomyosin structure organization and biogenesis; protein amino acid demethylation
Research Articles on ALKBH4
Similar Products
Product Notes
The ALKBH4 alkbh4 (Catalog #AAA389188) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ALKBH4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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