Western Blot (WB)
(Western blot analysis of ALKBH3 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). ALKBH3 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse ALKBH3 Polyclonal Antibody | anti-ALKBH3 antibody
ALKBH3, CT (Alpha-ketoglutarate-dependent Dioxygenase alkB Homolog 3, Alkylated DNA Repair Protein alkB Homolog 3, Prostate Cancer Antigen 1, DEPC-1, DEPC1, ABH3) (PE)
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of ALKBH3 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). ALKBH3 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ALKBH3 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). ALKBH3 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ALKBH3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ALKBH3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]
Uniprot Description
ALKBH3: Dioxygenase that repairs alkylated DNA containing 1- methyladenine (1meA) and 3-methylcytosine (3meC) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated 3mC within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKHB3. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Belongs to the alkB family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Demethylase; DNA repair, damage; Hydrolase; EC 1.14.11.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 11p11.2
Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus
Molecular Function: protein binding; L-ascorbic acid binding; ferrous iron binding; DNA-N1-methyladenine dioxygenase activity; DNA demethylase activity
Biological Process: cell proliferation; DNA dealkylation; DNA repair
Research Articles on ALKBH3
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Product Notes
The ALKBH3 alkbh3 (Catalog #AAA6462500) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALKBH3, CT (Alpha-ketoglutarate-dependent Dioxygenase alkB Homolog 3, Alkylated DNA Repair Protein alkB Homolog 3, Prostate Cancer Antigen 1, DEPC-1, DEPC1, ABH3) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ALKBH3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-1:500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ALKBH3 alkbh3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALKBH3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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