Rabbit ALDH2 Polyclonal Antibody | anti-ALDH2 antibody

ALDH2 Antibody (Center)

Cat. Num. AAA841276

• Gene Names: ALDH2; ALDM; ALDHI; ALDH-E2
• Reactivity: Human. Predicted cross reactivity with Mouse and Rat samples.
• Applications: Western Blot, Immunohistochemistry
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: ALDH2; Polyclonal Antibody; ALDH2 Antibody (Center); ALDM; Aldehyde dehydrogenase; mitochondrial; ALDH class 2; ALDH-E2; ALDHI.; anti-ALDH2 antibody

• Host: Rabbit
• Reactivity: Human. Predicted cross reactivity with Mouse and Rat samples.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: 100 ul of antibody in PBS with 0.09% (W/V) sodium azide; Appearance: Colorless liquid
• Sequence Length: 517

• Applicable Applications for anti-ALDH2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blot: ~1:1000, IHC: ~1:10-1:50.
• Immunogen: This ALDH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-347 amino acids from the Central region of human ALDH2.
• Positive Control: Western blot: A549 cell lysates and mouse liver and lung and rat liver tissue lysates, IHC: Human hepatocarcinoma.
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-ALDH2 antibody

Polyclonal Antibody to detect ALDH2 in human, mouse and rat samples

Background: ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH1A1, also designated retinal dehydrogenase 1 (RalDH1 or RALDH1); aldehyde dehydrogenase family 1 member A1; aldehyde dehydrogenase cytosolic; ALDHII; ALDH-E1 or ALDH E1, is a retinal dehydrogenase that participates in the biosynthesis of retinoic acid (RA). The major liver isoform ALDH1 localizes to cytosolic space, while ALDH2 localizes to the mitochondria. The ALDH1A2 (RALDH2, RALDH2-T) gene produces three different transcripts and also catalyzes the synthesis of RA from retinaldehyde. ALDH2 is present in most Caucasians, yet is absent in 50% of Asians. The absence of this enzyme has been linked to alcohol intolerance; and thusly, a reduced risk for alcoholism-related liver disease.

Product Categories/Family for anti-ALDH2 antibody

Antibodies & Supporting Tools; Antibodies for Metabolic Processes (NEW) Diabetes; Obesity & Metabolic Syndrome; Antibodies (NEW) Antibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 25777732
• NCBI GeneID: 217
• NCBI Accession #: NP_000681.2
• NCBI GenBank Nucleotide #: NM_000690.3
• UniProt Accession #: P05091; Q03639; Q6IB13; Q6IV71; B4DW54; E7EUE5
• Molecular Weight: 50,989 Da
• NCBI Official Full Name: aldehyde dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 2 family (mitochondrial)
• NCBI Official Symbol: ALDH2
• NCBI Official Synonym Symbols: ALDM; ALDHI; ALDH-E2
• NCBI Protein Information: aldehyde dehydrogenase, mitochondrial
• UniProt Protein Name: Aldehyde dehydrogenase, mitochondrial
• UniProt Gene Name: ALDH2
• UniProt Synonym Gene Names: ALDM
• UniProt Entry Name: ALDH2_HUMAN

NCBI Description

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]

Uniprot Description

ALDH2: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]

Protein type: Amino Acid Metabolism - histidine; Oxidoreductase; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Other Amino Acids Metabolism - beta-alanine; Lipid Metabolism - fatty acid; EC 1.2.1.3; Lipid Metabolism - glycerolipid; Amino Acid Metabolism - arginine and proline; Carbohydrate Metabolism - ascorbate and aldarate; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - tryptophan; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - propanoate; Secondary Metabolites Metabolism - limonene and pinene degradation

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: mitochondrial matrix

Molecular Function: aldehyde dehydrogenase (NAD) activity; electron carrier activity; aldehyde dehydrogenase [NAD(P)+] activity

Biological Process: synaptic transmission; ethanol catabolic process; xenobiotic metabolic process; alcohol metabolic process; carbohydrate metabolic process; ethanol oxidation; neurotransmitter biosynthetic process

Disease: Alcohol Sensitivity, Acute