Rabbit anti-Human ALDH2 Polyclonal Antibody | anti-ALDH2 antibody

ALDH2 (Aldehyde Dehydrogenase, Mitochondrial, ALDH Class 2, ALDH-E2, ALDHI, ALDM) (AP)

Cat. Num. AAA6369440

• Gene Names: ALDH2; ALDM; ALDHI; ALDH-E2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ALDH2; Polyclonal Antibody; ALDH2 (Aldehyde Dehydrogenase; Mitochondrial; ALDH Class 2; ALDH-E2; ALDHI; ALDM) (AP); anti-ALDH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALDH2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-ALDH2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human ALDH2, aa1-517 (NP_000681.2).
• Immunogen Sequence: MLRAAARFGPRLGRRLLSAAATQAVPAPNQQPEVFCNQIFINNEWHDAVSRKTFPTVNPSTGEVICQVAEGDKEDVDKAVKAARAAFQLGSPWRRMDASHRGRLLNRLADLIERDRTYLAALETLDNGKPYVISYLVDLDMVLKCLRYYAGWADKYHGKTIPIDGDFFSYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVNIVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQVAAGSSNLKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQSPFGGYKMSGSGRELGEYGLQAYTEVKTVTVKVPQKNS
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ALDH2 rabbit polyclonal antibody. Western Blot analysis of ALDH2 expression in human liver.)

Western Blot (WB)

(Western Blot analysis of ALDH2 expression in transfected 293T cell line by ALDH2 polyclonal antibody. Lane 1: ALDH2 transfected lysate (56.4kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-ALDH2 antibody

ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Asians have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Asians than among Caucasians could be related to the absence of the mitochondrial isozyme.

Product Categories/Family for anti-ALDH2 antibody

Antibodies; Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 25777732
• NCBI GeneID: 217
• NCBI Accession #: NP_000681.2
• NCBI GenBank Nucleotide #: NM_000690.2
• UniProt Accession #: P05091; Q03639; Q6IB13; Q6IV71; B4DW54; E7EUE5
• Molecular Weight: 50,989 Da
• NCBI Official Full Name: aldehyde dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 2 family (mitochondrial)
• NCBI Official Symbol: ALDH2
• NCBI Official Synonym Symbols: ALDM; ALDHI; ALDH-E2
• NCBI Protein Information: aldehyde dehydrogenase, mitochondrial; ALDH class 2; acetaldehyde dehydrogenase 2; liver mitochondrial ALDH; nucleus-encoded mitochondrial aldehyde dehydrogenase 2
• UniProt Protein Name: Aldehyde dehydrogenase, mitochondrial
• UniProt Gene Name: ALDH2
• UniProt Synonym Gene Names: ALDM
• UniProt Entry Name: ALDH2_HUMAN

NCBI Description

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]

Uniprot Description

ALDH2: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]

Protein type: Lipid Metabolism - glycerolipid; Lipid Metabolism - fatty acid; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 1.2.1.3; Amino Acid Metabolism - histidine; Carbohydrate Metabolism - ascorbate and aldarate; Mitochondrial; Carbohydrate Metabolism - propanoate; Oxidoreductase; Amino Acid Metabolism - arginine and proline; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - tryptophan; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - butanoate

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: mitochondrial matrix

Molecular Function: aldehyde dehydrogenase (NAD) activity; electron carrier activity; aldehyde dehydrogenase [NAD(P)+] activity

Biological Process: synaptic transmission; ethanol catabolic process; xenobiotic metabolic process; alcohol metabolic process; carbohydrate metabolic process; ethanol oxidation; neurotransmitter biosynthetic process

Disease: Alcohol Sensitivity, Acute