Rabbit NXF1 Polyclonal Antibody | anti-NXF1 antibody

NXF1 (Nuclear RNA Export Factor 1, TAP, MEX67) (HRP)

Cat. Num. AAA6430053

• Gene Names: NXF1; TAP; MEX67
• Reactivity: Human, Mouse, Rat
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: NXF1; Polyclonal Antibody; NXF1 (Nuclear RNA Export Factor 1; TAP; MEX67) (HRP); anti-NXF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes NXF1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-NXF1 antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 1:20-1:50; WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human NXF1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NXF1 antibody.)

Product Categories/Family for anti-NXF1 antibody

Antibodies; Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 125625324
• NCBI GeneID: 10482
• NCBI Accession #: NP_001074960.1
• NCBI GenBank Nucleotide #: NM_001081491.1
• UniProt Accession #: Q9UBU9; Q99799; Q9UQL2; B4E269
• Molecular Weight: 70,182 Da
• NCBI Official Full Name: nuclear RNA export factor 1 isoform 2
• NCBI Official Synonym Full Names: nuclear RNA export factor 1
• NCBI Official Symbol: NXF1
• NCBI Official Synonym Symbols: TAP; MEX67
• NCBI Protein Information: nuclear RNA export factor 1; mRNA export factor TAP; tip-associated protein; tip associating protein; tip-associating protein
• UniProt Protein Name: Nuclear RNA export factor 1
• Protein Family: Nuclear RNA export factor
• UniProt Gene Name: NXF1
• UniProt Synonym Gene Names: TAP
• UniProt Entry Name: NXF1_HUMAN

NCBI Description

This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

NXF1: Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5. Belongs to the NXF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 11q12-q13

Cellular Component: nucleoplasm; nuclear speck; nuclear pore; cytosol; nuclear inclusion body

Molecular Function: mRNA binding; protein binding; nucleotide binding; nucleocytoplasmic transporter activity

Biological Process: mRNA export from nucleus; poly(A)+ mRNA export from nucleus; viral reproduction; gene expression