Rabbit anti-Human ALDH1A1 Polyclonal Antibody | anti-ALDH1A1 antibody

ALDH1A1, ID (Retinal Dehydrogenase 1, RalDH1, RALDH 1, Aldehyde Dehydrogenase Family 1 Member A1, Aldehyde Dehydrogenase, Cytosolic, ALHDII, ALDH-E1, ALDH1A1, ALDC, ALDH1, PUMB1)

Cat. Num. AAA627327

• Gene Names: ALDH1A1; ALDC; ALDH1; PUMB1; ALDH11; RALDH1; ALDH-E1; MGC2318
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ALDH1A1; Polyclonal Antibody; ID (Retinal Dehydrogenase 1; RalDH1; RALDH 1; Aldehyde Dehydrogenase Family 1 Member A1; Aldehyde Dehydrogenase; Cytosolic; ALHDII; ALDH-E1; ALDC; ALDH1; PUMB1); Anti -ALDH1A1; anti-ALDH1A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALDH1A1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-ALDH1A1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:50; Immunofluorescence: 1:10-1:50
• Immunogen: Synthetic peptide selected from the center region of human ALDH1A1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ALDH1A1 (arrow) using MBS627327. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted using MBS627327, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of in HeLa cells using MBS627327. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells using MBS627327 (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ALDH1A1 antibody

ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.

Product Categories/Family for anti-ALDH1A1 antibody

Antibodies; Abs to Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 55960696
• NCBI GeneID: 216
• UniProt Accession #: Q5SYQ7; P00352; Q59EJ0; Q5SYQ8; Q5SYQ9
• Molecular Weight: 22,654 Da
• NCBI Official Full Name: aldehyde dehydrogenase 1 family, member A1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 1 family, member A1
• NCBI Official Symbol: ALDH1A1
• NCBI Official Synonym Symbols: ALDC; ALDH1; PUMB1; ALDH11; RALDH1; ALDH-E1; MGC2318
• NCBI Protein Information: retinal dehydrogenase 1; ALHDII; RALDH 1; ALDH class 1; OTTHUMP00000021482; acetaldehyde dehydrogenase 1; retinaldehyde dehydrogenase 1; aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase, liver cytosolic
• UniProt Protein Name: Aldehyde dehydrogenase 1 family, member A1
• Protein Family: Retinal dehydrogenase
• UniProt Gene Name: ALDH1A1
• UniProt Entry Name: Q5SYQ7_HUMAN

NCBI Description

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq]

Uniprot Description

ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.

Protein type: Cofactor and Vitamin Metabolism - retinol; Apoptosis; EC 1.2.1.36; GAPs; GAPs, Ras; Oxidoreductase

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: cytoplasm; cytosol

Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; 3-chloroallyl aldehyde dehydrogenase activity; benzaldehyde dehydrogenase (NAD+) activity; retinal dehydrogenase activity

Biological Process: retinol metabolic process; xenobiotic metabolic process; aldehyde metabolic process; ethanol oxidation