Western Blot (WB)
(Western Blot analysis of lysates (35ug/lane) from HepG2 cell line and human lung tissue lysate (from left to right), using MBS624608 (1:1000/lane). An IgG H&L (HRP) goat anti-rabbit (1:5000) was used as the secondary antibody.)
Rabbit anti-Human ALDH1A1 Polyclonal Antibody | anti-ALDH1A1 antibody
ALDH1A1 (Retinal Dehydrogenase 1, RalDH1, RALDH 1, Aldehyde Dehydrogenase Family 1 Member A1, Aldehyde Dehydrogenase, Cytosolic, ALHDII, ALDH-E1, ALDH1A1, ALDC, ALDH1, PUMB1)
Purified by Protein A affinity chromatography
Purified by Protein A affinity chromatography
Western Blot: 1:1000
Immunofluorescence: 1:100
Flow Cytometry: 1:10-1:50
Immunohistochemistry (FFPE): 1:50-1:100
Optimal dilutions to be dtermined by the researcher.
Western Blot (WB)
(Western Blot analysis of lysates (35ug/lane) from HepG2 cell line and human lung tissue lysate (from left to right), using MBS624608 (1:1000/lane). An IgG H&L (HRP) goat anti-rabbit (1:5000) was used as the secondary antibody.)
Western Blot (WB)
(Western Blot analysis of lysates (35ug/lane) from HepG2 cell line and human lung tissue lysate (from left to right), using MBS624608 (1:1000/lane). An IgG H&L (HRP) goat anti-rabbit (1:5000) was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using MBS624608 followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using MBS624608 followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Testing Data
Testing Data
Immunofluorescence (IF)
(Fluorescent confocal image of HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS624608 (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10ug/ml, 5 min). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.)
Immunofluorescence (IF)
(Fluorescent confocal image of HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS624608 (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10ug/ml, 5 min). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq]
Uniprot Description
ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.
Protein type: GAPs; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; Apoptosis; EC 1.2.1.36; GAPs, Ras
Chromosomal Location of Human Ortholog: 9q21.13
Cellular Component: cytoplasm; cytosol
Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; 3-chloroallyl aldehyde dehydrogenase activity; benzaldehyde dehydrogenase (NAD+) activity; retinal dehydrogenase activity
Biological Process: retinol metabolic process; xenobiotic metabolic process; aldehyde metabolic process; ethanol oxidation
Research Articles on ALDH1A1
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Product Notes
The ALDH1A1 aldh1a1 (Catalog #AAA624608) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ALDH1A1 (Retinal Dehydrogenase 1, RalDH1, RALDH 1, Aldehyde Dehydrogenase Family 1 Member A1, Aldehyde Dehydrogenase, Cytosolic, ALHDII, ALDH-E1, ALDH1A1, ALDC, ALDH1, PUMB1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALDH1A1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF), Flow Cyotmetry (FC/FACS), Immunohistochemistry (IHC). Other applications not tested. ELISA: 1:1,000 Western Blot: 1:1000 Immunofluorescence: 1:100 Flow Cytometry: 1:10-1:50 Immunohistochemistry (FFPE): 1:50-1:100 Optimal dilutions to be dtermined by the researcher. Researchers should empirically determine the suitability of the ALDH1A1 aldh1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALDH1A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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