Western Blot (WB)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. chemiluminescence.)
Goat Aldehyde Reductase Polyclonal Antibody | anti-AKR1A1 antibody
Goat anti-Aldehyde Reductase Antibody
Immunohistochemistry: In paraffin embedded Human Kidney shows Brush Border staining in PCT. Recommended concentration, 2-3ug/ml.
Western Blot: Approx 37kDa band observed in Human Liver and Human Placenta lysates. In transfected HEK293 transiently expressing Human AKR1A1 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. (calculated MW of 36.6kDa according to NP_006057.1 and NP_697021.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing AKR1A1 (RC200302) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing AKR1A1 (RC200302) and probed (mock transfection in first lane), tested by Origene.)
Immunohistochemistry (IHC)
((2.5g/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((2.5g/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of this gene exist, all encoding the same protein. [provided by RefSeq, Jan 2011]
Uniprot Description
AKR1A1: Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4- nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D- glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Belongs to the aldo/keto reductase family.
Protein type: Oxidoreductase; Lipid Metabolism - glycerolipid; EC 1.1.1.2; Carbohydrate Metabolism - glycolysis and gluconeogenesis
Chromosomal Location of Human Ortholog: 1p33-p32
Cellular Component: apical plasma membrane; cytosol; extracellular space
Molecular Function: aldehyde reductase activity; aldo-keto reductase activity; electron carrier activity; L-glucuronate reductase activity; protein binding
Biological Process: aldehyde catabolic process; aldehyde metabolic process; D-glucuronate catabolic process; glucose metabolic process; glucuronate catabolic process to xylulose 5-phosphate; L-ascorbic acid biosynthetic process
Research Articles on AKR1A1
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Product Notes
The AKR1A1 akr1a1 (Catalog #AAA421266) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Aldehyde Reductase Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Aldehyde Reductase can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Kidney shows Brush Border staining in PCT. Recommended concentration, 2-3ug/ml. Western Blot: Approx 37kDa band observed in Human Liver and Human Placenta lysates. In transfected HEK293 transiently expressing Human AKR1A1 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. (calculated MW of 36.6kDa according to NP_006057.1 and NP_697021.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the AKR1A1 akr1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DAGHPLYPFN DPY. It is sometimes possible for the material contained within the vial of "Aldehyde Reductase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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