Western Blot (WB)
(Western blot analysis of HGFAC using NIH-3T3 whole cell lysates)
Rabbit HGFAC Polyclonal Antibody | anti-HGFAC antibody
HGFAC Antibody
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
IHC: 1:200
IF/ICC: 1:100-1:500
Western Blot (WB)
(Western blot analysis of HGFAC using NIH-3T3 whole cell lysates)
Western Blot (WB)
(Western blot analysis of HGFAC using NIH-3T3 whole cell lysates)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using HGFAC Antibody. Lane 1: c476 treated with blocking peptide. Lane 2: c476; Lane 3: 293; )
Western Blot (WB)
(Western blot analysis of extracts from various samples, using HGFAC Antibody. Lane 1: c476 treated with blocking peptide. Lane 2: c476; Lane 3: 293; )
Immunofluorescence (IF)
(MBS9609887 staining HEPG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600 was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9609887 staining HEPG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600 was used as secondary antibody.)
Immunohistochemistry (IHC)
(MBS9609887 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609887 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Subunit Structure: Heterodimer of a short chain and a long chain linked by a disulfide bond.
Similarity: Belongs to the peptidase S1 family.
NCBI and Uniprot Product Information
Predicted: 71 kDa
NCBI Description
This gene encodes a member of the peptidase S1 protein family. The encoded protein is first synthesized as an inactive single-chain precursor before being activated to a heterodimeric form by endoproteolytic processing. It acts as serine protease that converts hepatocyte growth factor to the active form. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form.CautionIt is uncertain whether Met-1 is the initiator.
Research Articles on HGFAC
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Product Notes
The HGFAC hgfac (Catalog #AAA9609887) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HGFAC Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HGFAC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the HGFAC hgfac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HGFAC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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