Rabbit anti-Human SFRS1 Polyclonal Antibody | anti-SFRS1 antibody

SFRS1, CT (Splicing Factor, Arginine/Serine-rich 1, pre-mRNA-splicing Factor SF2, P33 Subunit, Alternative-splicing Factor 1, ASF-1, OK/SW-cl.3, SF2P33, SF2, ASF) (MaxLight 550)

Cat. Num. AAA6500520

• Gene Names: SRSF1; ASF; SF2; SFRS1; SF2p33; SRp30a
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: SFRS1; Polyclonal Antibody; CT (Splicing Factor; Arginine/Serine-rich 1; pre-mRNA-splicing Factor SF2; P33 Subunit; Alternative-splicing Factor 1; ASF-1; OK/SW-cl.3; SF2P33; SF2; ASF) (MaxLight 550); anti-SFRS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SFRS1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-SFRS1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human SFRS1 (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SFRS1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). SFRS1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SFRS1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of SFRS1 (arrow) using rabbit polyclonal SFRS1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SFRS1 gene.)

Flow Cytometry (FC/FACS)

(SFRS1 Antibody (C-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SFRS1 antibody

Antibodies; Splicing Factors

References

1. Sugiyama, N., et.al., Mol. Cell Proteomics 6(6), 1103-1109 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 5902076
• NCBI GeneID: 6426
• NCBI Accession #: NP_008855
• NCBI GenBank Nucleotide #: NM_006924.4
• UniProt Accession #: Q07955; Q13809; B2R6Z7; D3DTZ3
• Molecular Weight: 29.9 kDa (268aa)
• NCBI Official Full Name: serine/arginine-rich splicing factor 1 isoform 1
• NCBI Official Synonym Full Names: serine and arginine rich splicing factor 1
• NCBI Official Symbol: SRSF1
• NCBI Official Synonym Symbols: ASF; SF2; SFRS1; SF2p33; SRp30a
• NCBI Protein Information: serine/arginine-rich splicing factor 1
• UniProt Protein Name: Serine/arginine-rich splicing factor 1
• UniProt Gene Name: SRSF1
• UniProt Synonym Gene Names: ASF; SF2; SF2P33; SFRS1; ASF-1
• UniProt Entry Name: SRSF1_HUMAN

NCBI Description

This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13. [provided by RefSeq, Jun 2014]

Uniprot Description

SF2: a serine-arginine-rich splicing regulatory protein. Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5' and 3' splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice-site-containing pre-mRNA. Extensively phosphorylated on serine residues in the serine-arginine rich region. Three splice variant isoforms have been described. ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.

Protein type: RNA-binding; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus

Molecular Function: mRNA binding; protein binding; RS domain binding; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; in utero embryonic development; RNA splicing; regulation of mRNA stability; regulation of translation; nuclear mRNA splicing, via spliceosome; nuclear mRNA 5'-splice site recognition; mRNA splice site selection; regulation of nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; regulation of transcription, DNA-dependent; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; cardiac muscle contraction