Rabbit anti-Mouse AKT3 Polyclonal Antibody | anti-AKT3 antibody

AKT3 Antibody: Alkaline Phosphatase

Cat. Num. AAA8001282

• Gene Names: AKT3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: AKT3; Polyclonal Antibody; AKT3 Antibody: Alkaline Phosphatase; Rabbit Anti-Human AKT3 Polyclonal; RAC-gamma serine/threonine-protein kinase Antibody; AKT3 kinase Antibody; STK2 Antibody; V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma Antibody; V akt murine thymoma viral oncogene homolog 3 Antibody; MPPH Antibody; PKB gamma Antibody; STK-2 Antibody; Akt3 Antibody; STK 2 Antibody; RAC gamma serine/threonine protein kinase Antibody; AKT3_HUMAN Antibody; V akt murine thymoma viral oncogene homolog 3 (protein kinase B; gamma) Antibody; RACPK Gamma Antibody; AKT3 Antibody; Protein kinase Akt-3 Antibody; PRKBG Antibody; PKBG Antibody; Protein Kinase AKT3 Antibody; RAC-PK-gamma Antibody; DKFZp434N0250 Antibody; RAC gamma Antibody; Serine threonine protein kinase Akt3 Antibody; Serine threonine protein kinase Akt 3 Antibody; EC:2.7.11.1 Antibody; Protein kinase B gamma Antibody; anti-AKT3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Detects 124 kDa.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 465

• Applicable Applications for anti-AKT3 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Target: AKT3
• Immunogen: Synthetic peptide from the mid-protein of Human AKT3 (aa. 100-200)
• Immunogen Species: Human
• Cellular Localization: Nucleus; Cytoplasm; Membrane; Peripheral Membrane Protein
• Conjugate: Alkaline Phosphatase
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Tissue Specificity: In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Mouse Brain showing detection of ~55 kDa AKT3 protein using Rabbit Anti-AKT3 Polyclonal Antibody (SPC-769). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AKT3 Polyclonal Antibody (SPC-769) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55 kDa.)

Product Categories/Family for anti-AKT3 antibody

Antibodies; Cell Signaling; Protein Phosphorylation; Serine/Threonine Kinases; PKB/AKT; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 332078559
• NCBI GeneID: 10000
• NCBI Accession #: NP_001193658.1
• NCBI GenBank Nucleotide #: NM_001206729.1
• UniProt Accession #: Q9Y243; Q0VAA6; Q5VTI1; Q5VTI2; Q96QV3; Q9UFP5
• Molecular Weight: 54,032 Da
• NCBI Official Full Name: RAC-gamma serine/threonine-protein kinase isoform 2
• NCBI Official Synonym Full Names: AKT serine/threonine kinase 3
• NCBI Official Symbol: AKT3
• NCBI Official Synonym Symbols: MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
• NCBI Protein Information: RAC-gamma serine/threonine-protein kinase
• UniProt Protein Name: RAC-gamma serine/threonine-protein kinase
• Protein Family: RAC-gamma serine/threonine-protein kinase
• UniProt Gene Name: AKT3
• UniProt Synonym Gene Names: PKBG; PKB gamma

NCBI Description

The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.