Rabbit anti-Human AKAP5 Polyclonal Antibody | anti-AKAP5 antibody

AKAP5, ID (AKAP5, AKAP79, A-kinase anchor protein 5, A-kinase anchor protein 79kD, H21, cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein) (Biotin)

Cat. Num. AAA6272478

• Gene Names: AKAP5; H21; AKAP75; AKAP79
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AKAP5; Polyclonal Antibody; ID (AKAP5; AKAP79; A-kinase anchor protein 5; A-kinase anchor protein 79kD; H21; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein) (Biotin); cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein; anti-AKAP5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-AKAP5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AKAP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-143 amino acids from the Central region of human AKAP5.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AKAP5 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the AKAP5 antibody detected the AKAP5 protein (arrow).)

Related Product Information for anti-AKAP5 antibody

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT.

Product Categories/Family for anti-AKAP5 antibody

Antibodies; Protein Kinases

References

Willoughby, D., et al. J. Biol. Chem. 285(26):20328-20342(2010). Chen, M.H., et al. Cell. Signal. 21(1):136-142(2009). Tavalin, S.J. J. Biol. Chem. 283(17):11445-11452(2008). Correia, S.S., et al. Nat. Neurosci. 11(4):457-466(2008). Chai, S., et al. J. Biol. Chem. 282(31):22668-22677(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 158187521
• NCBI GeneID: 9495
• NCBI Accession #: NP_004848.3
• NCBI GenBank Nucleotide #: NM_004857.3
• UniProt Accession #: P24588; A2RRB8
• Molecular Weight: 47,088 Da
• NCBI Official Full Name: A-kinase anchor protein 5
• NCBI Official Synonym Full Names: A kinase (PRKA) anchor protein 5
• NCBI Official Symbol: AKAP5
• NCBI Official Synonym Symbols: H21; AKAP75; AKAP79
• NCBI Protein Information: A-kinase anchor protein 5; AKAP-5; AKAP 79; A-kinase anchor protein 79 kDa; A-kinase anchor protein, 79kDa; A-kinase anchoring protein 75/79; cAMP-dependent protein kinase regulatory subunit II high affinity binding protein; cAMP-dependent protein kinase
• UniProt Protein Name: A-kinase anchor protein 5
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP5
• UniProt Synonym Gene Names: AKAP79; AKAP-5; AKAP 79
• UniProt Entry Name: AKAP5_HUMAN

NCBI Description

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq, Jul 2008]

Uniprot Description

AKAP5: May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade. Binding protein for dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (PKA) and also for the protein kinase C (PKC) and the phosphatase calcineurin (PP2B). Each enzyme is inhibited when bound to the anchoring protein. Also binds the beta2-adrenergic receptor. Part of a complex containing AKAP5, ADCY5, ADCY6 AND PDE4C. Interacts with ADCY8, and enhances its phosphorylation at lipid rafts. Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior pituitary.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: plasma membrane; cytosol

Molecular Function: calmodulin binding; protein binding; protein kinase A binding; adenylate cyclase binding

Biological Process: synaptic transmission; positive regulation of cAMP biosynthetic process; energy reserve metabolic process; signal transduction; regulation of insulin secretion; protein targeting