Rabbit anti-Human, Rat HIST1H2BC Polyclonal Antibody | anti-HIST1H2BC antibody

HIST1H2BC(Ab-15) Antibody

Cat. Num. AAA7110711

• Gene Names: HIST1H2BG; H2B/a; H2BFA; H2B.1A; dJ221C16.8
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: HIST1H2BC; Polyclonal Antibody; HIST1H2BC(Ab-15) Antibody; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l; H2BFL; AND; HIST1H2BE; H2BFH; HIST1H2BF; H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; anti-HIST1H2BC antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 126

• Applicable Applications for anti-HIST1H2BC antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:5000; IHC: 1:10-1:100
• Immunogen: Peptide sequence around site of acetyl-Lys(15) derived from human Histone H2B
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Rat spleen tissueAll lanes: HIST1H2BC antibody at 0.76ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 14 KDaObserved band size: 14 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7110711 diluted at 1:20 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Product Categories/Family for anti-HIST1H2BC antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4504257
• NCBI GeneID: 8339
• NCBI Accession #: NP_003509.1
• NCBI GenBank Nucleotide #: NM_003518.3
• UniProt Accession #: P62807; P02278; Q3B872; Q4VB69; Q93078; Q93080
• Molecular Weight: 13,906 Da
• NCBI Official Full Name: histone H2B type 1-C/E/F/G/I
• NCBI Official Synonym Full Names: histone cluster 1 H2B family member g
• NCBI Official Symbol: HIST1H2BG
• NCBI Official Synonym Symbols: H2B/a; H2BFA; H2B.1A; dJ221C16.8
• NCBI Protein Information: histone H2B type 1-C/E/F/G/I
• UniProt Protein Name: Histone H2B type 1-C/E/F/G/I
• Protein Family: Histone
• UniProt Gene Name: HIST1H2BC
• UniProt Synonym Gene Names: H2BFL; H2B/a; H2B/g; H2B/h; H2B/k; H2B/l

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Uniprot Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.