Rabbit anti-Human, Mouse AK5 Polyclonal Antibody | anti-AK5 antibody

AK5, NT (Adenylate Kinase Isoenzyme 5, AK 5, ATP-AMP Transphosphorylase 5) (Azide free) (HRP)

Cat. Num. AAA6461679

• Gene Names: AK5; AK6
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: AK5; Polyclonal Antibody; NT (Adenylate Kinase Isoenzyme 5; AK 5; ATP-AMP Transphosphorylase 5) (Azide free) (HRP); anti-AK5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AK5. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-AK5 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human AK5 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-AK5 Pab is used in Western blot to detect AK5 in mouse liver tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with AK5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-AK5 antibody

Antibodies; Enzymes

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Van Rompay, A.R., et al., Eur. J. Biochem. 261(2):509-517 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 28144899
• NCBI GeneID: 26289
• NCBI Accession #: NP_036225.2
• NCBI GenBank Nucleotide #: NM_012093.3
• UniProt Accession #: Q9Y6K8; Q5U622; Q6FH66; Q7Z4T5; Q86YS0; Q8N464; Q96EC9
• Molecular Weight: 60,315 Da
• NCBI Official Full Name: adenylate kinase isoenzyme 5 isoform 2
• NCBI Official Synonym Full Names: adenylate kinase 5
• NCBI Official Symbol: AK5
• NCBI Official Synonym Symbols: AK6
• NCBI Protein Information: adenylate kinase isoenzyme 5; AK 5; ATP-AMP transphosphorylase 5; adenylate kinase 6
• UniProt Protein Name: Adenylate kinase isoenzyme 5
• Protein Family: Adenylate kinase
• UniProt Gene Name: AK5
• UniProt Synonym Gene Names: AK 5
• UniProt Entry Name: KAD5_HUMAN

NCBI Description

This gene encodes a member of the adenylate kinase family, which is involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. This member is related to the UMP/CMP kinase of several species. It is located in the cytosol and expressed exclusively in brain. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AK5: Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Belongs to the adenylate kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.4.6; Nucleotide Metabolism - purine; EC 2.7.4.3; Kinase, other

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: cytoplasm; microtubule organizing center; cytosol

Molecular Function: nucleoside kinase activity; nucleoside diphosphate kinase activity; adenylate kinase activity; ATP binding

Biological Process: ATP metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; nucleoside triphosphate biosynthetic process; pyrimidine ribonucleotide biosynthetic process; ADP biosynthetic process; nucleoside diphosphate phosphorylation; dADP biosynthetic process