Human Cytochrome c oxidase subunit 5A, mitochondrial ELISA Kit | COX5A elisa kit

Human Cytochrome c oxidase subunit 5A, mitochondrial ELISA Kit

Cat. Num. AAA760845

• Gene Names: COX5A; VA; COX; COX-VA
• Reactivity: Human
• Synonyms: Cytochrome c oxidase subunit 5A; mitochondrial; Human Cytochrome c oxidase subunit 5A; mitochondrial ELISA Kit; COX5A/Cytochrome c oxidase subunit 5A; mitochondrial/Cytochrome c oxidase polypeptide Va; COX5A elisa kit

• Reactivity: Human
• Sequence Length: 150

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for COX5A elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human COX5A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human COX5A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human COX5A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human COX5A can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 190885499
• NCBI GeneID: 9377
• NCBI Accession #: NP_004246.2
• NCBI GenBank Nucleotide #: NM_004255.3
• Molecular Weight: 16,762 Da
• NCBI Official Full Name: cytochrome c oxidase subunit 5A, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit 5A
• NCBI Official Symbol: COX5A
• NCBI Official Synonym Symbols: VA; COX; COX-VA
• NCBI Protein Information: cytochrome c oxidase subunit 5A, mitochondrial
• UniProt Protein Name: Cytochrome c oxidase subunit 5A, mitochondrial
• Protein Family: Cytochrome c oxidase
• UniProt Gene Name: COX5A
• UniProt Entry Name: COX5A_HUMAN

NCBI Description

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq, Jul 2008]

Uniprot Description

COX5A: This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase subunit 5A family.

Protein type: Energy Metabolism - oxidative phosphorylation; Mitochondrial; EC 1.9.3.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex IV; myelin sheath

Molecular Function: cytochrome-c oxidase activity; electron carrier activity; metal ion binding; protein binding

Biological Process: cellular metabolic process; gene expression; mitochondrial electron transport, cytochrome c to oxygen; transcription initiation from RNA polymerase II promoter