Rabbit anti-Human AK2 Polyclonal Antibody | anti-AK2 antibody

AK2, CT (Adenylate Kinase Isoenzyme 2, AK 2, ATP-AMP Transphosphorylase 2, ADK2) (FITC)

Cat. Num. AAA6461645

• Gene Names: AK2; ADK2; AK 2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: AK2; Polyclonal Antibody; CT (Adenylate Kinase Isoenzyme 2; AK 2; ATP-AMP Transphosphorylase 2; ADK2) (FITC); anti-AK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AK2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-AK2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human AK2 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-AK2 Pab is used in Western blot to detect AK2 in Jurkat cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-AK2 antibody

Antibodies; Enzymes

References

1. Noma, T., et al., Biochim. Biophys. Acta 1395(1):34-39 (1998). 2. Lee, Y., et al., J. Biochem. 123(1):47-54 (1998). 3. Lee, Y., et al., Biochem. Mol. Biol. Int. 39(4):833-842 (1996). 4. Bruns, G.A., et al., Biochem. Genet. 15(5-6), 477-486 (1977).

NCBI and Uniprot Product Information

• NCBI GI #: 1708596
• NCBI GeneID: 204
• UniProt Accession #: P54819; Q16856; Q5EB54; Q5TIF7; Q8TCY2; Q8TCY3; A8K6L1; B4DHH7; B4DL64
• Molecular Weight: 232
• NCBI Official Full Name: Adenylate kinase 2, mitochondrial
• NCBI Official Synonym Full Names: adenylate kinase 2
• NCBI Official Symbol: AK2
• NCBI Official Synonym Symbols: ADK2; AK 2
• NCBI Protein Information: adenylate kinase 2, mitochondrial; AK2; AK 2; ATP:AMP phosphotransferase; ATP-AMP transphosphorylase 2; adenylate monophosphate kinase; adenylate kinase isoenzyme 2, mitochondrial
• UniProt Protein Name: Adenylate kinase 2, mitochondrial
• UniProt Gene Name: AK2
• UniProt Entry Name: KAD2_HUMAN

NCBI Description

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.[provided by RefSeq, Nov 2010]

Uniprot Description

AK2: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis. Defects in AK2 are the cause of reticular dysgenesis (RDYS); also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1. Belongs to the adenylate kinase family. AK2 subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; Mitochondrial; EC 2.7.4.3; Kinase, other

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: mitochondrial inner membrane; mitochondrial intermembrane space; cytosol

Molecular Function: adenylate kinase activity; ATP binding

Biological Process: dATP metabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; ADP biosynthetic process; brain development; AMP metabolic process; liver development; oxidative phosphorylation; nucleotide phosphorylation

Disease: Reticular Dysgenesis