Western Blot (WB)
(Western Blot analysis of EIF2C3 expression in transfected 293T cell line by EIF2C3 polyclonal antibody. Lane 1: EIF2C3 transfected lysate (71.2kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human AGO3 Polyclonal Antibody | anti-Ago3 antibody
AGO3 (Protein Argonaute-3, Argonaute3, hAgo3, Eukaryotic Translation Initiation Factor 2C 3, eIF-2C 3, eIF2C 3, EIF2C3, FLJ12765, MGC86946)
Gene Names
Ago3; Piwi; Siwi2; BmAGO3
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
AGO3; Polyclonal Antibody; AGO3 (Protein Argonaute-3; Argonaute3; hAgo3; Eukaryotic Translation Initiation Factor 2C 3; eIF-2C 3; eIF2C 3; EIF2C3; FLJ12765; MGC86946); Anti -AGO3 (Protein Argonaute-3; anti-Ago3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human EIF2C3.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA
Applicable Applications for anti-Ago3 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human EIF2C3, aa1-626 (NP_803171.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of EIF2C3 expression in transfected 293T cell line by EIF2C3 polyclonal antibody. Lane 1: EIF2C3 transfected lysate (71.2kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of EIF2C3 expression in transfected 293T cell line by EIF2C3 polyclonal antibody. Lane 1: EIF2C3 transfected lysate (71.2kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-Ago3 antibody
Eukaryotic translation initiation factor 2C 3, Ago3, belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. Ago3 is indicated to play a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. Unlike Ago1 and Ago2, Ago3 and AG04 do not possess the ability to dissociate miRNA duplexes in a minimal in vitro system. Re-introduction of any Ago variant into embyronic (ES) cells deficient for expression of all four Ago variants will rescue miRNA silencing defects and reduce apoptosis.
Product Categories/Family for anti-Ago3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
argonaute 3
NCBI Official Symbol
Ago3
NCBI Official Synonym Symbols
Piwi; Siwi2; BmAGO3
NCBI Protein Information
argonaute 3
Protein Family
Research Articles on Ago3
Similar Products
Product Notes
The Ago3 (Catalog #AAA644200) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AGO3 (Protein Argonaute-3, Argonaute3, hAgo3, Eukaryotic Translation Initiation Factor 2C 3, eIF-2C 3, eIF2C 3, EIF2C3, FLJ12765, MGC86946) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AGO3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the Ago3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MCEVLDIHNI DEQPRPLTDS HRVKFTKEIK GLKVEVTHCG TMRRKYRVCN VTRRPASHQT FPLQLENGQT VERTVAQYFR EKYTLQLKYP HLPCLQVGQE QKHTYLPLEV CNIVAGQRCI KKLTDNQTST MIKATARSAP DRQEEISRLV RSANYETDPF VQEFQFKVRD EMAHVTGRVL PAPMLQYGGR NRTVATPSHG VWDMRGKQFH TGVEIKMWAI ACFATQRQCR EEILKGFTDQ LRKISKDAGM PIQGQPCFCK YAQGADSVEP MFRHLKNTYS GLQLIIVILP GKTPVYAEVK RVGDTLLGMA TQCVQVKNVI KTSPQTLSNL CLKINVKLGG INNILVPHQR PSVFQQPVIF LGADVTHPPA GDGKKPSIAA VVGSMDAHPS RYCATVRVQR PRQEIIQDLA SMVRELLIQF YKSTRFKPTR IIFYRDGVSE GQFRQVLYYE LLAIREACIS LEKDYQPGIT YIVVQKRHHT RLFCADRTER VGRSGNIPAG TTVDTDITHP YEFDFYLCSH AGIQGTSRPS HYHVLWDDNC FTADELQLLT YQLCHTYVRC TRSVSIPAPA YYAHLVAFRA RYHLVDKEHD SAEGSHVSGQ SNGRDPQALA KAVQIHQDTL RTMYFA. It is sometimes possible for the material contained within the vial of "AGO3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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