Rabbit anti-Human Adenylate Kinase 1 Polyclonal Antibody | anti-AK1 antibody

Adenylate Kinase 1 (Adenylate Kinase Isoenzyme 1, AK 1, AK1, AK-1, Adenylate Kinase Soluble, ATP-AMP Transphosphorylase, Myokinase) (APC)

Cat. Num. AAA6461610

• Gene Names: AK1; HTL-S-58j
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Adenylate Kinase 1; Polyclonal Antibody; Adenylate Kinase 1 (Adenylate Kinase Isoenzyme 1; AK 1; AK1; AK-1; Adenylate Kinase Soluble; ATP-AMP Transphosphorylase; Myokinase) (APC); anti-AK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AK1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-AK1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human AK1 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-AK1 Pab in Jurkat cell line lysate (35ug/lane). AK1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of AK1 (arrow) using rabbit polyclonal AK1 Antibody (S45). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AK1 gene.)

Product Categories/Family for anti-AK1 antibody

Antibodies; Enzymes

References

1. Corrons, J.L., et al., Blood 102(1):353-356 (2003). 2. Toren, A., et al., Br. J. Haematol. 87(2):376-380 (1994). 3. Zuffardi, O., et al., Hum. Genet. 82(1):17-19 (1989). 4. Matsuura, S., et al., J. Biol. Chem. 264(17):10148-10155 (1989). 5. Miwa, S., et al., Am. J. Hematol. 14(4):325-333 (1983).

NCBI and Uniprot Product Information

• NCBI GI #: 4502011
• NCBI GeneID: 203
• NCBI Accession #: NP_000467
• NCBI GenBank Nucleotide #: NM_000476.2
• UniProt Accession #: P00568; Q9BVK9; Q9UQC7
• Molecular Weight: 21,635 Da
• NCBI Official Full Name: adenylate kinase isoenzyme 1 isoform 1
• NCBI Official Synonym Full Names: adenylate kinase 1
• NCBI Official Symbol: AK1
• NCBI Official Synonym Symbols: HTL-S-58j
• NCBI Protein Information: adenylate kinase isoenzyme 1
• UniProt Protein Name: Adenylate kinase isoenzyme 1
• Protein Family: Adenylate kinase
• UniProt Gene Name: AK1
• UniProt Synonym Gene Names: AK 1
• UniProt Entry Name: KAD1_HUMAN

NCBI Description

This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Uniprot Description

AK1: Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Defects in AK1 are the cause of hemolytic anemia due to adenylate kinase deficiency (HAAKD). Belongs to the adenylate kinase family.

Protein type: Kinase, other; EC 2.7.4.3; EC 2.7.4.6; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: cytoplasm; plasma membrane; cytosol; outer dense fiber

Molecular Function: nucleoside diphosphate kinase activity; adenylate kinase activity; ATP binding

Biological Process: ATP metabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleoside triphosphate biosynthetic process; nucleoside diphosphate phosphorylation; ADP biosynthetic process; AMP metabolic process; cell cycle arrest

Disease: Adenylate Kinase Deficiency, Hemolytic Anemia Due To