SDS-Page
(Recombinant AGO3 protein gel. AGO3 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)
AGO3 recombinant protein
Recombinant AGO3 protein
Gene Names
AGO3; EIF2C3
Purity
The AGO3 protein is >75% pure by SDS-PAGE.
Synonyms
AGO3; Recombinant AGO3 protein; Argonaute 3; RISC Catalytic Component; Eukaryotic Translation Factor 2C; EIF2C3; HAgo3; Argonaute3; EIF-2C; EIF2C; AGO3 recombinant protein
Host
Baculovirus
Purity/Purification
The AGO3 protein is >75% pure by SDS-PAGE.
Form/Format
Recombinant full length AGO3 protein was expressed in Sf9 cells and is provided in 25mM Tris pH8.0, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mg/ml 3X DYKDDDDK peptide.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant AGO3 is suitable for use in the study of miRNA screening, RNAi research and selectivity profiling. This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant AGO3 protein gel. AGO3 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)
SDS-Page
(Recombinant AGO3 protein gel. AGO3 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)
Related Product Information for AGO3 recombinant protein
Short Description: Recombinant human AGO3 was expressed in a baculovirus expression system as the full-length protein (accession number NP_079128.2) with an N-terminal DYKDDDDK-Tag. The molecular weight of the protein is 100.4 kDa. The AGO3 protein is >75% pure by SDS-PAGE. Recombinant AGO3 is suitable for use in the study of miRNA screening, RNAi research and selectivity profiling.
Background: AGO3 (argonaute RISC catalytic component 3, also known as EIF2C3) is a member of the Argonaute family of proteins which play a role in RNA interference. The Argonaute family proteins (AGO1, AGO2, AGO3 and AGO4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it recognizes a complementary strand, it activates AGO2 to cleaves the mRNA. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. In some cases, RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). AGO3 lacks endonuclease activity and does not appear to cleave target mRNAs. AGO3 contain a PAZ and a PIWI domain.
Background: AGO3 (argonaute RISC catalytic component 3, also known as EIF2C3) is a member of the Argonaute family of proteins which play a role in RNA interference. The Argonaute family proteins (AGO1, AGO2, AGO3 and AGO4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it recognizes a complementary strand, it activates AGO2 to cleaves the mRNA. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. In some cases, RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). AGO3 lacks endonuclease activity and does not appear to cleave target mRNAs. AGO3 contain a PAZ and a PIWI domain.
Product Categories/Family for AGO3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,360 Da
NCBI Official Full Name
protein argonaute-3 isoform a
NCBI Official Synonym Full Names
argonaute RISC catalytic component 3
NCBI Official Symbol
AGO3
NCBI Official Synonym Symbols
EIF2C3
NCBI Protein Information
protein argonaute-3; hAgo3; eIF2C 3; eIF-2C 3; argonaute3; argonaute 3; eukaryotic translation initiation factor 2C, 3
UniProt Protein Name
Protein argonaute-3
Protein Family
UniProt Gene Name
AGO3
UniProt Synonym Gene Names
EIF2C3; Argonaute3; hAgo3; eIF-2C 3; eIF2C 3
UniProt Entry Name
AGO3_HUMAN
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Product Notes
The AGO3 ago3 (Catalog #AAA388227) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "AGO3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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