Rabbit aFGF Polyclonal Antibody | anti-aFGF antibody
Rabbit anti aFGF Polyclonal
Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months
• requirement of phosphatidylinositol 3-kinase activity for translocation of exogenous aFGF to the cytosol and nucleus .J. Biol. Chem., Apr 2000; 275: 11972.
• angiogenic potential of perivascularly delivered aFGF in a porcine model of chronic myocardial ischemia .Am J Physiol Heart Circ Physiol, Mar 1998; 274: H930 - H936.
• the fibroblast growth factor receptor-1 is necessary for the induction of neurite outgrowth in pc12 cells by aFGF .J. Neurosci., Aug 1996; 16: 4579.
• the major acidic fibroblast growth factor (aFGF) -stimulated phosphoprotein from bovine liver plasma membranes has aFGF-stimulated kinase, autoadenylylation, and alkaline nucleotide phosphodiesterase activities .J. Biol. Chem., Dec 1993; 268: 27318 - 27326.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
Uniprot Description
FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; Cytokine
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: nucleoplasm; proteinaceous extracellular matrix; extracellular space; nucleolus; extracellular region; cell cortex; cytosol
Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; fibroblast growth factor receptor binding
Biological Process: epidermal growth factor receptor signaling pathway; anatomical structure morphogenesis; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; multicellular organismal development; positive regulation of cholesterol biosynthetic process; signal transduction; positive regulation of MAP kinase activity; positive regulation of angiogenesis; positive regulation of cell division; induction of an organ; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of epithelial cell proliferation; positive regulation of cell migration; lung development
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Product Notes
The aFGF fgf1 (Catalog #AAA551012) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti aFGF Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's aFGF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA). ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Researchers should empirically determine the suitability of the aFGF fgf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "aFGF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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