Typical Testing Data/Standard Curve (for reference only)
(Human FGF1 PicoKine ELISA Kit standard curve )
Human FGF1 ELISA Kit | FGF1 elisa kit
Human FGF1 PicoKine ELISA Kit
O.D.: |0.007|0.069|0.161|0.359|0.670|1.288|1.955|2.224
n: |16|16|16|24|24|24
Mean(pg/ml): |83.4|288|1204|95.1|302|1357
Standard Deviation: |3.67|16.13|71.04|5.90|20.54|100.42
CV(%): |4.4|5.6|5.9|6.2|6.8|7.4
Typical Testing Data/Standard Curve (for reference only)
(Human FGF1 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human FGF1 PicoKine ELISA Kit standard curve )
Background: Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.
Principle: Human FGF1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for FGF1 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: A2-D155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FGF1 amount of sample captured in plate.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
Uniprot Description
FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation; Cytokine; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 5q31.3
Cellular Component: cytosol; extracellular region; extracellular space; nucleoplasm
Molecular Function: 1-phosphatidylinositol-3-kinase activity; fibroblast growth factor receptor binding; growth factor activity; heparin binding; integrin binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity
Biological Process: activation of MAPK activity; activation of protein kinase B; anatomical structure morphogenesis; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; multicellular organismal development; phosphoinositide-mediated signaling; positive regulation of angiogenesis; positive regulation of cell division; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cholesterol biosynthetic process; positive regulation of MAP kinase activity; positive regulation of transcription from RNA polymerase II promoter; regulation of phosphoinositide 3-kinase cascade
Research Articles on FGF1
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Product Notes
The Human FGF1 fgf1 (Catalog #AAA178716) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178716 ELISA Kit recognizes Human FGF1. It is sometimes possible for the material contained within the vial of "FGF1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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