FGF1 active protein

Recombinant Mouse FGF1

Cat. Num. AAA4159917

• Gene Names: Fgf1; Fam; Fgfa; Dffrx; Fgf-1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: FGF1; Recombinant Mouse FGF1; aFGF; Fibroblast growth factor 1; FGF-1; Heparin-binding growth factor 1; HBGF-1; Recombinant Mouse Acidic Fibroblast Growth Factor (FGF1); FGF1 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered PBS solution.
• Sequence Length: 155

• Bioactivity: The ED(50) as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <= 10 ng/mL, corresponding to a specific activity of >= 1.0 x 10^5 units/mg.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for FGF1 active protein

aFGF and bFGF display 55% homology which is limited, however, to a few functional domains. The structure of aFGF, revealed by X-ray crystallography, shows a similar folding structure as IL-1. Because aFGF binds to the same receptor as bFGF, aFGF displays more or less the same spectrum of activities. It is, however, generally less active than bFGF in similar assays. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 6753850
• NCBI GeneID: 14164
• NCBI Accession #: NP_034327.1
• NCBI GenBank Nucleotide #: NP_034327.1
• UniProt Accession #: P61148
• Molecular Weight: 16.0 kDa
• NCBI Official Full Name: fibroblast growth factor 1
• NCBI Official Synonym Full Names: fibroblast growth factor 1
• NCBI Official Symbol: Fgf1
• NCBI Official Synonym Symbols: Fam; Fgfa; Dffrx; Fgf-1
• NCBI Protein Information: fibroblast growth factor 1
• UniProt Protein Name: Fibroblast growth factor 1
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf1
• UniProt Synonym Gene Names: Fgf-1; Fgfa; FGF-1; aFGF; HBGF-1
• UniProt Entry Name: FGF1_MOUSE

Uniprot Description

FGF1: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1. Part of a Cu(2+)-dependent multiprotein aggregate containing FGF1, S100A13 and SYT1. Interacts with SYT1. Interacts with S100A13. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Cytokine; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; extracellular space; proteinaceous extracellular matrix; cytoplasm; nucleolus; extracellular region; nucleus; cytosol

Molecular Function: heparin binding; protein binding; growth factor activity; Hsp70 protein binding; receptor binding; fibroblast growth factor receptor binding

Biological Process: fibroblast growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of cholesterol biosynthetic process; cardiac muscle cell proliferation; positive regulation of MAP kinase activity; cell proliferation; positive regulation of angiogenesis; induction of an organ; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; angiogenesis; cell differentiation; positive regulation of epithelial cell proliferation; lung development; positive regulation of cell migration