Western Blot (WB)
(Western blot analysis of AFAP1L1 in A549 cell lysate with AFAP1L1 antibody at 1 μg/mL.)
Rabbit anti-Human, Mouse AFAP1L Polyclonal Antibody | anti-AFAP1L1 antibody
AFAP1L Antibody
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
AFAP1L Antibody is affinity chromatography purified via peptide column.
Synonyms
AFAP1L; Polyclonal Antibody; AFAP1L Antibody; Actin filament-associated protein 1-like 1; AFAP1-like protein 1; actin filament associated protein 1-like 1; anti-AFAP1L1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
At least four isoforms of AFAP1L1 are known to exist. This antibody is predicted to not cross-react with other AFAP family members.
Purity/Purification
AFAP1L Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
768
Applicable Applications for anti-AFAP1L1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
AFAP1L1 antibody can be used for detection of AFAP1L1 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
AFAP1L1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human AFAP1L1.
Buffer
AFAP1L Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
AFAP1L antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of AFAP1L1 in A549 cell lysate with AFAP1L1 antibody at 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of AFAP1L1 in A549 cell lysate with AFAP1L1 antibody at 1 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of AFAP1L in A549 cells with AFAP1L antibody at 5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of AFAP1L in A549 cells with AFAP1L antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of AFAP1L in A549 cells with AFAP1L antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of AFAP1L in A549 cells with AFAP1L antibody at 20 μg/mL.)
Related Product Information for anti-AFAP1L1 antibody
AFAP1L Antibody: The actin filament-associated protein 1 (AFAP1)-like 1 protein is one of three members in the AFAP1 family of adaptor proteins. Like AFAP1, AFAP1L1 is an actin binding protein that has a role in actin cytoskeleton arrangement, but AFAP1L1 is thought to have a unique function distinct from AFAP1. AFAP1L1 colocalizes with cortactin and localizes to invadosomes, highly dynamic, actin-rich adhesion structures harboring metalloproteases, suggesting that AFAP1L1 affects invadosome formation (1, 4). Recent reports suggest that AFAP1L1 can be used as a prognostic marker for spindle cell sarcomas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,455 Da
NCBI Official Full Name
actin filament-associated protein 1-like 1 isoform 1
NCBI Official Synonym Full Names
actin filament associated protein 1-like 1
NCBI Official Symbol
AFAP1L1
NCBI Protein Information
actin filament-associated protein 1-like 1; AFAP1-like protein 1
UniProt Protein Name
Actin filament-associated protein 1-like 1
Protein Family
UniProt Gene Name
AFAP1L1
UniProt Synonym Gene Names
AFAP1-like protein 1
UniProt Entry Name
AF1L1_HUMAN
Uniprot Description
AFAP1L1: a protein containing two pleckstrin homology (PH) domains, which mediate protein-protein and protein-lipid interactions.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 5q32
Cellular Component: cytoplasm; plasma membrane; podosome; cell junction; nucleus
Research Articles on AFAP1L1
Similar Products
Product Notes
The AFAP1L1 afap1l1 (Catalog #AAA150104) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AFAP1L Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AFAP1L can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). AFAP1L1 antibody can be used for detection of AFAP1L1 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the AFAP1L1 afap1l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AFAP1L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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