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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ADRM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Rabbit ADRM1 Polyclonal Antibody | anti-ADRM1 antibody

ADRM1 Polyclonal Antibody

Gene Names
ADRM1; ARM1; ARM-1; GP110
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity Purification
Synonyms
ADRM1; Polyclonal Antibody; ADRM1 Polyclonal Antibody; ARM1; ARM-1; GP110; anti-ADRM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
407
Applicable Applications for anti-ADRM1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IP: 1:20-1:50
IHC: 1:50-1:200
IF: 1:50-1:200
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human ADRM1
Calculated Molecular Weight
42kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ADRM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ADRM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Related Product Information for anti-ADRM1 antibody
This gene encodes a member of the adhesion regulating molecule 1 protein family. The encoded protein is a component of the proteasome where it acts as a ubiquitin receptor and recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Increased levels of the encoded protein are associated with increased cell adhesion, which is likely an indirect effect of this intracellular protein. Dysregulation of this gene has been implicated in carcinogenesis. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-ADRM1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
407
NCBI Official Full Name
Proteasomal ubiquitin receptor ADRM1
NCBI Official Synonym Full Names
adhesion regulating molecule 1
NCBI Official Symbol
ADRM1
NCBI Official Synonym Symbols
ARM1; ARM-1; GP110
NCBI Protein Information
proteasomal ubiquitin receptor ADRM1; rpn13 homolog; M(r) 110,000 surface antigen; 110 kDa cell membrane glycoprotein; proteasome regulatory particle non-ATPase 13
UniProt Protein Name
Proteasomal ubiquitin receptor ADRM1
UniProt Gene Name
ADRM1
UniProt Synonym Gene Names
GP110; Gp110; ARM-1; hRpn13
UniProt Entry Name
ADRM1_HUMAN

NCBI Description

This gene encodes a member of the adhesion regulating molecule 1 protein family. The encoded protein is a component of the proteasome where it acts as a ubiquitin receptor and recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Increased levels of the encoded protein are associated with increased cell adhesion, which is likely an indirect effect of this intracellular protein. Dysregulation of this gene has been implicated in carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

ADRM1: Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity. Interacts with PSMD1, ubiquitin and UCHL5. Belongs to the ADRM1 family.

Protein type: Proteasome complex; Cell development/differentiation

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: proteasome complex; nucleoplasm; membrane; integral to plasma membrane; cytoplasm; plasma membrane

Molecular Function: protein binding; protease binding

Biological Process: RNA elongation from RNA polymerase II promoter; proteasome assembly

Research Articles on ADRM1

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Product Notes

The ADRM1 adrm1 (Catalog #AAA129216) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADRM1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADRM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IP: 1:20-1:50 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the ADRM1 adrm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADRM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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