Rabbit anti-Human ADRM1 Polyclonal Antibody | anti-ADRM1 antibody

ADRM1, CT (ADRM1, GP110, Proteasomal ubiquitin receptor ADRM1, 110kD cell membrane glycoprotein, Adhesion-regulating molecule 1, Proteasome regulatory particle non-ATPase 13, Rpn13 homolog) (PE)

Cat. Num. AAA6271958

• Gene Names: ADRM1; ARM1; ARM-1; GP110
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADRM1; Polyclonal Antibody; CT (ADRM1; GP110; Proteasomal ubiquitin receptor ADRM1; 110kD cell membrane glycoprotein; Adhesion-regulating molecule 1; Proteasome regulatory particle non-ATPase 13; Rpn13 homolog) (PE); Rpn13 homolog; anti-ADRM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ADRM1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ADRM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368-397 amino acids from the C-terminal region of human ADRM1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ADRM1 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ADRM1 antibody detected the ADRM1 protein (arrow).)

Related Product Information for anti-ADRM1 antibody

The protein encoded by this gene is an integral plasma membrane protein which promotes cell adhesion. The encoded protein is thought to undergo O-linked glycosylation. Expression of this gene has been shown to be induced by gamma interferon in some cancer cells. Two transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-ADRM1 antibody

Antibodies; Ubiquitin

References

Mazumdar, T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(31):13854-13859(2010). Elangovan, M., et al. Biochem. Biophys. Res. Commun. 396(2):425-428(2010). Chen, X., et al. Mol. Cell 38(3):404-415(2010). Kim, T., et al. Biochem. Biophys. Res. Commun. 390(3):585-590(2009). Chen, W., et al. Zhonghua Zhong Liu Za Zhi 31(11):815-819(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 28373192
• NCBI GeneID: 11047
• NCBI Accession #: NP_008933.2
• NCBI GenBank Nucleotide #: NM_007002.3
• UniProt Accession #: Q16186; Q96FJ7; Q9H1P2; A0PKB1
• Molecular Weight: 42,153 Da
• NCBI Official Full Name: proteasomal ubiquitin receptor ADRM1 isoform 1
• NCBI Official Synonym Full Names: adhesion regulating molecule 1
• NCBI Official Symbol: ADRM1
• NCBI Official Synonym Symbols: ARM1; ARM-1; GP110
• NCBI Protein Information: proteasomal ubiquitin receptor ADRM1; rpn13 homolog; M(r) 110,000 surface antigen; 110 kDa cell membrane glycoprotein; proteasome regulatory particle non-ATPase 13
• UniProt Protein Name: Proteasomal ubiquitin receptor ADRM1
• UniProt Gene Name: ADRM1
• UniProt Synonym Gene Names: GP110; Gp110; ARM-1; hRpn13
• UniProt Entry Name: ADRM1_HUMAN

NCBI Description

This gene encodes a member of the adhesion regulating molecule 1 protein family. The encoded protein is a component of the proteasome where it acts as a ubiquitin receptor and recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Increased levels of the encoded protein are associated with increased cell adhesion, which is likely an indirect effect of this intracellular protein. Dysregulation of this gene has been implicated in carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

ADRM1: Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity. Interacts with PSMD1, ubiquitin and UCHL5. Belongs to the ADRM1 family.

Protein type: Proteasome complex; Cell development/differentiation

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: proteasome complex; nucleoplasm; membrane; integral to plasma membrane; cytoplasm; plasma membrane

Molecular Function: protein binding; protease binding

Biological Process: RNA elongation from RNA polymerase II promoter; proteasome assembly