Rabbit anti-Human, Mouse ADH4 Polyclonal Antibody | anti-ADH4 antibody

ADH4, CT (ADH4, Alcohol dehydrogenase 4, Alcohol dehydrogenase class II pi chain) (Biotin)

Cat. Num. AAA6271752

• Gene Names: ADH4; ADH-2; HEL-S-4
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADH4; Polyclonal Antibody; CT (ADH4; Alcohol dehydrogenase 4; Alcohol dehydrogenase class II pi chain) (Biotin); Alcohol dehydrogenase class II pi chain; anti-ADH4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ADH4 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ADH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-356 amino acids from the C-terminal region of human ADH4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ADH4 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ADH4 antibody detected the ADH4 protein (arrow).)

Western Blot (WB)

(Western blot analysis of ADH4 (arrow) using rabbit polyclonal ADH4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH4 gene.)

Immunohistochemistry (IHC)

(ADH4 antibody(C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH4 antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ADH4 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ADH4 antibody

This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.

Product Categories/Family for anti-ADH4 antibody

Antibodies; Enzymes; Dehydrogenase

References

Pochareddy, S., et al. Gene 460 (1-2), 1-7 (2010): Preuss, U.W., et al. Addict Biol (2010). In press: Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Rainero, I., et al. Headache 50(1):92-98(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 71565152
• NCBI GeneID: 127
• NCBI Accession #: NP_000661.2
• NCBI GenBank Nucleotide #: NM_000670.4
• UniProt Accession #: P08319; Q8TCD7; A8K470; B4DIE7; C9J4A9
• Molecular Weight: 42,607 Da
• NCBI Official Full Name: alcohol dehydrogenase 4 isoform 2
• NCBI Official Synonym Full Names: alcohol dehydrogenase 4 (class II), pi polypeptide
• NCBI Official Symbol: ADH4
• NCBI Official Synonym Symbols: ADH-2; HEL-S-4
• NCBI Protein Information: alcohol dehydrogenase 4
• UniProt Protein Name: Alcohol dehydrogenase 4
• Protein Family: Alcohol dehydrogenase
• UniProt Gene Name: ADH4

NCBI Description

This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq, Jul 2008]

Uniprot Description

ADH4: class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq, Jul 2008]

Protein type: Amino Acid Metabolism - tyrosine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Cofactor and Vitamin Metabolism - retinol; EC 1.1.1.1; Lipid Metabolism - fatty acid; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: alcohol dehydrogenase activity; alcohol dehydrogenase activity, zinc-dependent; all-trans retinal binding; benzaldehyde dehydrogenase activity; NAD binding; NADPH:quinone reductase activity; oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; retinol binding; retinol dehydrogenase activity; zinc ion binding

Biological Process: alcohol catabolic process; alcohol metabolic process; aldehyde metabolic process; ethanol oxidation; retinoid metabolic process; retinol metabolic process