Rabbit IRS-1 (Ab-312) Polyclonal Antibody | anti-IRS-1 antibody

Rabbit IRS-1 (Ab-312) Antibody

Cat. Num. AAA2122

• Gene Names: IRS1; HIRS-1
• Reactivity: Human,Mouse,Rat,Monkey
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
• Synonyms: IRS-1 (Ab-312); Polyclonal Antibody; Rabbit IRS-1 (Ab-312) Antibody; anti-IRS-1 antibody

• Host: Rabbit
• Reactivity: Human,Mouse,Rat,Monkey
• Clonality: Polyclonal
• Specificity: IRS-1 (Ab-312) detects endogenous levels of total IRS-1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1242

• Applicable Applications for anti-IRS-1 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Western Blot (WB) 1:500-1:2000; Immunohistochemistry (IHC) 1:50-1:200; Immunofluorescence/Immunocytochemistry (IF/ICC) 1:100-1:500
• Immunogen: A synthesized peptide derived from human IRS-1.
• Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Preparation and Storage: Store at -20°C.Stable for 12 months from date of receipt.

Western Blot

(Western blot analysis of IRS-1 expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.)

Testing Data (TD)

(Anti-diabetes effect of chronic intermittent hypobaric hypoxia through improving liver insulin resistance in diabetic rats YM Tian, Y Liu, S Wang, Y Dong, T Su, HJ Ma, Y Zhang Life sciences, 2016 Elsevier)

Related Product Information for anti-IRS-1 antibody

IRS-1 is an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiplevtyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2.

NCBI and Uniprot Product Information

• NCBI GI #: 5031805
• NCBI GeneID: 3667
• NCBI Accession #: NP_005535.1
• NCBI GenBank Nucleotide #: NM_005544.2
• UniProt Accession #: P35568
• Molecular Weight: 180 kDa
• NCBI Official Full Name: insulin receptor substrate 1
• NCBI Official Synonym Full Names: insulin receptor substrate 1
• NCBI Official Symbol: IRS1
• NCBI Official Synonym Symbols: HIRS-1
• NCBI Protein Information: insulin receptor substrate 1; IRS-1
• UniProt Protein Name: Insulin receptor substrate 1
• UniProt Gene Name: IRS1
• UniProt Synonym Gene Names: IRS-1
• UniProt Entry Name: IRS1_HUMAN

NCBI Description

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Uniprot Description

IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q36

Cellular Component: intracellular membrane-bound organelle; cytoplasm; plasma membrane; caveola; cytosol; nucleus

Molecular Function: insulin-like growth factor receptor binding; protein binding; signal transducer activity; protein kinase C binding; phosphoinositide 3-kinase binding; SH2 domain binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding

Biological Process: response to peptide hormone stimulus; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; glucose homeostasis; signal transduction; response to insulin stimulus; negative regulation of insulin secretion; positive regulation of glucose import; mammary gland development; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; protein kinase B signaling cascade; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of phosphoinositide 3-kinase activity; positive regulation of fatty acid beta-oxidation; cellular response to insulin stimulus; insulin-like growth factor receptor signaling pathway; regulation of gene expression; insulin receptor signaling pathway; innate immune response; positive regulation of insulin receptor signaling pathway; lipid catabolic process; positive regulation of cell migration

Disease: Diabetes Mellitus, Noninsulin-dependent