Rabbit anti-Rat ADH1B Polyclonal Antibody | anti-ADH1B antibody

ADH1B Polyclonal Antibody

Cat. Num. AAA3008385

• Gene Names: ADH1B; ADH2; HEL-S-117
• Reactivity: Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ADH1B; Polyclonal Antibody; ADH1B Polyclonal Antibody; ADH2; Alcohol dehydrogenase 1B; anti-ADH1B antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ADH1B polyclonal antibody detects endogenous levels of ADH1B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 375

• Applicable Applications for anti-ADH1B antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human ADH1B.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of rat liver cells, using ADH1B antibody.)

Related Product Information for anti-ADH1B antibody

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID: 19365573). This antibody is specific to ADH1B.

NCBI and Uniprot Product Information

• NCBI GI #: 113394
• NCBI GeneID: 125
• UniProt Accession #: P00325
• Molecular Weight: ~ 40kDa
• NCBI Official Full Name: All-trans-retinol dehydrogenase
• NCBI Official Synonym Full Names: alcohol dehydrogenase 1B (class I), beta polypeptide
• NCBI Official Symbol: ADH1B
• NCBI Official Synonym Symbols: ADH2; HEL-S-117
• NCBI Protein Information: all-trans-retinol dehydrogenase [NAD(+)] ADH1B
• UniProt Protein Name: Alcohol dehydrogenase 1B
• UniProt Gene Name: ADH1B
• UniProt Synonym Gene Names: ADH2
• UniProt Entry Name: ADH1B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Uniprot Description

ADH1B: Dimer of identical or non-identical chains of three types; alpha, beta and gamma. Belongs to the zinc-containing alcohol dehydrogenase family.

Protein type: Lipid Metabolism - fatty acid; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.1; Oxidoreductase; Amino Acid Metabolism - tyrosine; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: zinc ion binding; alcohol dehydrogenase activity, zinc-dependent

Biological Process: xenobiotic metabolic process; ethanol oxidation

Disease: Alcohol Dependence