Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysate, Rat heart tissueAll lanes: ADGRA2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 143, 120, 20 KDaObserved band size: 143, 120 KDa)
Rabbit anti-Human, Rat ADGRA2 Polyclonal Antibody | anti-ADGRA2 antibody
ADGRA2 Antibody
Gene Names
ADGRA2; TEM5; GPR124
Reactivity
Human, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
ADGRA2; Polyclonal Antibody; ADGRA2 Antibody; Adhesion G protein-coupled receptor A2Imported; G-protein coupled receptor 124; anti-ADGRA2 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
1338
Applicable Applications for anti-ADGRA2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:200
IHC: 1:20-1:200
IF: 1:50-1:200
Immunogen
Recombinant Human Adhesion G protein-coupled receptor A2 protein (1069-1338AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysate, Rat heart tissueAll lanes: ADGRA2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 143, 120, 20 KDaObserved band size: 143, 120 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysate, Rat heart tissueAll lanes: ADGRA2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 143, 120, 20 KDaObserved band size: 143, 120 KDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7104665 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7104665 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunofluorescent analysis of HepG2 cells using MBS7104665 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC)
(Immunofluorescent analysis of HepG2 cells using MBS7104665 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-ADGRA2 antibody
Endothelial receptor which functions as a WNT7-specific coactivator of canonical Wnt signaling (By similarity). Required for normal endothelial cell sprouting and migration in the forebrain and neural tube (By similarity). Has a major role in establishing the blood-brain barrier (By similarity). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628).
Product Categories/Family for anti-ADGRA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,530 Da
NCBI Official Full Name
adhesion G protein-coupled receptor A2
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor A2
NCBI Official Symbol
ADGRA2
NCBI Official Synonym Symbols
TEM5; GPR124
NCBI Protein Information
adhesion G protein-coupled receptor A2
UniProt Protein Name
Adhesion G protein-coupled receptor A2
Protein Family
UniProt Gene Name
ADGRA2
Uniprot Description
Endothelial receptor which functions as a WNT7-specific coactivator of canonical Wnt signaling (). Required for normal endothelial cell sprouting and migration in the forebrain and neural tube (). Has a major role in establishing the blood-brain barrier (). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628).
Research Articles on ADGRA2
Similar Products
Product Notes
The ADGRA2 adgra2 (Catalog #AAA7104665) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADGRA2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADGRA2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the ADGRA2 adgra2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADGRA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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